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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC713.06
Gene ID 49/E03
Gene synonyms/obsolete
Gene product DNA ligase; similar to Sp CDC17
Entry clone Cloned
ORF length (unspliced) 2325
ORF length (spliced)
Entry clone length 2325
No. of intron 0
Sequence status Finished
Sequence results 1530T:A / 1669T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC713.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTCCCAAAAAGCGCAT
Rev primer name SPBC713.06.Rv
Rev primer SEQ AGAAAGCTGGGTAAGTATTAAGTTGTTTCATA
Amino acid length 774
Molecular weight 87.5
Isoelectric point (calc.) 7.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus; nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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