Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC20H4.04
Gene ID 49/E05
Gene synonyms/obsolete
Gene product DEAD/DEAH box helicase; ATP-dependent; RNA helicase; similar to Sp SPAC9.05
Entry clone Cloned
ORF length (unspliced) 2352
ORF length (spliced)
Entry clone length 2352
No. of intron 0
Sequence status Finished
Sequence results 297A:G / 953A:T / 1220T:C / 1588G:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC20H4.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATTGTACTGAGAGACTC
Rev primer name SPAC20H4.04.Rv
Rev primer SEQ AGAAAGCTGGGTATGTACTTTGAAATAATGCA
Amino acid length 783
Molecular weight 89.4
Isoelectric point (calc.) 9.8
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LHNKIQGLSL
Localization (YFP) nucleolus>nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.