Gene name |
SPBC216.01c |
Gene ID |
49/E10 |
Gene synonyms/obsolete |
SPBC713.13c |
Gene product |
eukaryotic conserved
protein |
Entry clone |
Cloned |
ORF length (unspliced) |
2569 |
ORF length (spliced) |
2511 |
Entry clone length |
2569 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
600T:C / 1143A:G /
1609T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC216.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATTCTACGAAGGAAGA |
Rev primer name |
SPBC216.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGAAGCGTAGCCATTCTGG |
Amino acid length |
836 |
Molecular weight |
95.4 |
Isoelectric point (calc.) |
4.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSTFSELLNI |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |