Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPBC691.03c
Gene ID 49/E12
Gene synonyms/obsolete apl3; pi033; SPACTOKYO_453.02c
Gene product adaptin; AP-2 adaptor complex; involved in vesicule-mediated transport; Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration; Alpha adaptin is a subunit of the plasma membrane adaptor
Entry clone Cloned
ORF length (unspliced) 2637
ORF length (spliced)
Entry clone length 2637
No. of intron 0
Sequence status Finished
Sequence results 433G:A / 666A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC691.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTGGCTAGCAACAATAT
Rev primer name SPBC691.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAAACGAATTCCTTAAAATC
Amino acid length 878
Molecular weight 100
Isoelectric point (calc.) 6.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LIGYLAVALLL/LIRVLDRILSL
Localization (YFP) cytosol=nucleus; cytoplasmic dots at periphery
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.