Gene name |
SPAPB24D3.10c |
Gene ID |
49/F05 |
Gene synonyms/obsolete |
agl1; agl |
Gene product |
alpha-glucosidase;
glycosyl hydrolase family 31; similar to Sp SPAC922.02c and
SPAC30D11.01c |
Entry clone |
Cloned |
ORF length (unspliced) |
2910 |
ORF length (spliced) |
|
Entry clone length |
2910 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
825T:C / 1014T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAPB24D3.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATGATTTCTACTGCCTA |
Rev primer name |
SPAPB24D3.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACAAAGTAAGATTCCAGCTG |
Amino acid length |
969 |
Molecular weight |
108.7 |
Isoelectric point (calc.) |
4.2 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LAETIHGLRL/LANVTILGL |
Localization (YFP) |
periphery |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |