Gene name |
SPAC110.01 |
Gene ID |
49/F07 |
Gene synonyms/obsolete |
SPAC140.05 |
Gene product |
putative
5'-AMP-activated protein kinase; serine/threonine protein
kinase |
Entry clone |
Cloned |
ORF length (unspliced) |
3072 |
ORF length (spliced) |
|
Entry clone length |
3072 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
155A:G / 936T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC110.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACGCTCAACCCTTTCA |
Rev primer name |
SPAC110.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAAAACGGTAGCGTGTCTC |
Amino acid length |
1023 |
Molecular weight |
114 |
Isoelectric point (calc.) |
10.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
periphery with
discontinuity |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |