Gene name |
SPAPB1A10.06c |
Gene ID |
49/G04 |
Gene synonyms/obsolete |
|
Gene product |
DEAD/DEAH box
helicase; ATP-dependent; RNA helicase; ribonucleoprotein (RNP)
complex; processome component; involved in rRNA processing
|
Entry clone |
Cloned# |
ORF length (unspliced) |
3599 |
ORF length (spliced) |
3552 |
Entry clone length |
3599 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
328C:G / 3284T:A /
3286G:T / 3298T:G / 3299T:G |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAPB1A10.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGGAGACTGCGTAAAAG |
Rev primer name |
SPAPB1A10.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACAATTCACCCAACGCCCA |
Amino acid length |
1183 |
Molecular weight |
133.9 |
Isoelectric point (calc.) |
7.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSPRFSKMLII |
Localization (YFP) |
nucleolus>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |