Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAPB2C8.01
Gene ID 49/G09
Gene synonyms/obsolete
Gene product Sp specific families; glycoprotein; threonine-rich protein; possibly Sp specific; 3kb of 36 AA repeat; agglutinin-like; similar to Sp SPBC1348.08C
Entry clone Cloned
ORF length (unspliced) 3663
ORF length (spliced)
Entry clone length 3663
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAPB2C8.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCAGTTTCCCGATTGCT
Rev primer name SPAPB2C8.01.Rv
Rev primer SEQ AGAAAGCTGGGTAGTTATCAATACCACCATTA
Amino acid length 1220
Molecular weight 124
Isoelectric point (calc.) 3.4
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ambiguous structure
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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