| Gene name |
SPAPB1E7.04c |
| Gene ID |
49/G10 |
| Gene synonyms/obsolete |
|
| Gene product |
glycosyl hydrolase
family 18; glucoamylase I (alpha-1,4-glucan glucosidase);
extracellular starch-degrading enzyme; chitinase family
signature |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3711 |
| ORF length (spliced) |
|
| Entry clone length |
3711 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1766G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAPB1E7.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGGTTAATATCTTCTTT |
| Rev primer name |
SPAPB1E7.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCGGCAGTGGTTGTTGCA |
| Amino acid length |
1236 |
| Molecular weight |
123.3 |
| Isoelectric point (calc.) |
4.3 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
vacuole |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |