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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP19A11.06
Gene ID 49/H05
Gene synonyms/obsolete lid2; SPBP4H10.01
Gene product Lid family protein; DNA-binding protein; zinc finger protein; zf-PHD finger; zf-C5HC2 type; jmjC domain; Arid/BRIGHT domain; similar domain organization to human retinoblastoma binding protein 2; involved in transcriptional regulation (implicated); interacts physically with SET1 complex; involved in regulation of chromatin remodeling (implicated); similar to Sp SPAC343.11c (paralog)
Entry clone Cloned
ORF length (unspliced) 4542
ORF length (spliced)
Entry clone length 4542
No. of intron 0
Sequence status Finished
Sequence results 2262A:G / 3601C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP19A11.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATATGCCAGCTTTTGTG
Rev primer name SPBP19A11.06.Rv
Rev primer SEQ AGAAAGCTGGGTATACATTTAGAAAATTCTCG
Amino acid length 1513
Molecular weight 172.2
Isoelectric point (calc.) 7.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LHKVIIYLRL/LVPNIQALKL/LGFTIPELGI
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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