Gene name |
SPBC32H8.13c |
Gene ID |
49/H11 |
Gene synonyms/obsolete |
mok12;
SPACTOKYO_453.25c |
Gene product |
a-1,3-glucan synthase;
alpha glucan synthase; glycosyl transferase family 1; similar
to Sp MOK1 and MOK11 and MOK13 and MOK14; no apparent Sc
ortholog; involved in cell wall biosynthesis |
Entry clone |
Cloned |
ORF length (unspliced) |
7059 |
ORF length (spliced) |
|
Entry clone length |
7059 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
3496T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC32H8.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTCTTCTTTAGCATACC |
Rev primer name |
SPBC32H8.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAGAGCAAGCTGACTTTTA |
Amino acid length |
2352 |
Molecular weight |
266.5 |
Isoelectric point (calc.) |
6.6 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
14 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFFSIPTILLI/LEYDVPDLGI/LWSIIILLLI/LLSVIKILSL/LTKSVWLLLAL |
Localization (YFP) |
Golgi; periphery |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |