| Gene name |
SPCC4G3.08 |
| Gene ID |
50/A06 |
| Gene synonyms/obsolete |
psk1 |
| Gene product |
serine/threonine
protein kinase; similar to Sp SPAC22E12.14C (paralog) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1311 |
| ORF length (spliced) |
|
| Entry clone length |
1311 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC4G3.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTGTCTTTATGTTTGA |
| Rev primer name |
SPCC4G3.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATGGTTCGAAACTGCGTTG |
| Amino acid length |
436 |
| Molecular weight |
49.3 |
| Isoelectric point (calc.) |
6.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIHLHKLGI/LHEEFGNLTI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
| Microscope used for
observation |
DeltaVision |