| Gene name |
SPAPB8E5.10 |
| Gene ID |
50/B03 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein;
sequence orphan; similar to D. melanogaster CG12569 (possibly
spurious) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
335 |
| ORF length (spliced) |
222 |
| Entry clone length |
335 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAPB8E5.10.Fd |
| Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGGCAGTTTTATCAAGAAT |
| Rev primer name |
SPAPB8E5.10.Rv |
| Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTAATGAAATTGCGGTTTCTTA |
| Amino acid length |
73 |
| Molecular weight |
8.4 |
| Isoelectric point (calc.) |
10.7 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPFELKPLAI |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |