| Gene name |
SPBC1105.18c |
| Gene ID |
50/B06 |
| Gene synonyms/obsolete |
SPBC887.21c |
| Gene product |
peptidyl tRNA
hydrolase; peptide release factor |
| Entry clone |
Cloned |
| ORF length (unspliced) |
489 |
| ORF length (spliced) |
|
| Entry clone length |
489 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
25T:C / 80T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1105.18.Fd |
| Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGCTGTGCGCAGCACGAAA |
| Rev primer name |
SPBC1105.18.Rv |
| Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTAGATCACGTCTTGTTTCGCT |
| Amino acid length |
162 |
| Molecular weight |
18.8 |
| Isoelectric point (calc.) |
10.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
127 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
DeltaVision |