Gene name |
SPAC20G4.01 |
Gene ID |
50/C01 |
Gene synonyms/obsolete |
SPAC22F8.13 |
Gene product |
CCR4-Not complex;
non-transporter with ATP binding cassette |
Entry clone |
Cloned |
ORF length (unspliced) |
967 |
ORF length (spliced) |
843 |
Entry clone length |
967 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC20G4.01.Fd |
Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGGAAGTAACTGTTTCAAA |
Rev primer name |
SPAC20G4.01.Rv |
Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTATTTTGCTCGGGAAATCTTA |
Amino acid length |
280 |
Molecular weight |
31.2 |
Isoelectric point (calc.) |
6.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLRPFEVLLL |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |