| Gene name |
SPBP22H7.09c |
| Gene ID |
50/F02 |
| Gene synonyms/obsolete |
pi022;
SPACTOKYO_453.12 |
| Gene product |
involved in chromosome
segregation |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1341 |
| ORF length (spliced) |
1236 |
| Entry clone length |
1341 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
66T:C / 104A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBP22H7.09.Fd |
| Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGCGTACTGAATTGTCTAG |
| Rev primer name |
SPBP22H7.09.Rv |
| Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTAGTTAATCTCTTGTTGTCCG |
| Amino acid length |
411 |
| Molecular weight |
47.7 |
| Isoelectric point (calc.) |
6.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGQIAQLEI/LPNPLVFLEL |
| Localization (YFP) |
SPB; nuclear dots;
nucleus |
| Comments for localization |
nuclear dots by over
expression? |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |