| Gene name |
SPBC32H8.06 |
| Gene ID |
50/F04 |
| Gene synonyms/obsolete |
pi018;
SPACTOKYO_453.18 |
| Gene product |
TPR repeat protein
|
| Entry clone |
Cloned |
| ORF length (unspliced) |
1365 |
| ORF length (spliced) |
1152 |
| Entry clone length |
1365 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
593A:G / 866A:G /
1255A:G / 1262A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC32H8.06.Fd |
| Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGGAAGAAAAGGAGAACGT |
| Rev primer name |
SPBC32H8.06.Rv |
| Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTATGTGTAGCAATTGATTGGG |
| Amino acid length |
383 |
| Molecular weight |
44.7 |
| Isoelectric point (calc.) |
6.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB; periphery at site
of septum formation; nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |