Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPBC713.12
Gene ID 50/F06
Gene synonyms/obsolete
Gene product squalene epoxidase; involved in ergosterol biosynthesis
Entry clone Cloned
ORF length (unspliced) 1374
ORF length (spliced)
Entry clone length 1374
No. of intron 0
Sequence status Finished
Sequence results 624A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC713.12.Fd
Fwd primer SEQ GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGGCAACTCAAGACGCTGA
Rev primer name SPBC713.12.Rv
Rev primer SEQ GGGGACCACTTTGTACAAGAAAGCTGGGTAGGATGACATTTCGCTAAGG
Amino acid length 457
Molecular weight 50.3
Isoelectric point (calc.) 8.5
Signal SEQ
No. of transmembrane domain 3
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLIFLQASLVI
Localization (YFP) ER; cytoplasmic dots; vacuole membrane by over expression
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.