Gene name |
SPBC32H8.11 |
Gene ID |
50/G12 |
Gene synonyms/obsolete |
mei4; pi013;
SPACTOKYO_453.23 |
Gene product |
transcription factor;
fork head domain; essential; required for meiosis;
autoregulated expression; similar to Sp sep1 (paralog) |
Entry clone |
Cloned |
ORF length (unspliced) |
1554 |
ORF length (spliced) |
|
Entry clone length |
1554 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC32H8.11.Fd |
Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGGTTGAAAATCAAGGGAA |
Rev primer name |
SPBC32H8.11.Rv |
Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTAAAGAGAGAAGTCATTGATA |
Amino acid length |
517 |
Molecular weight |
57.6 |
Isoelectric point (calc.) |
6.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |