| Gene name |
SPBC32H8.09 |
| Gene ID |
50/H03 |
| Gene synonyms/obsolete |
pi015;
SPACTOKYO_453.21c |
| Gene product |
WD repeat protein; no
apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1563 |
| ORF length (spliced) |
1452 |
| Entry clone length |
1563 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
135T:C / 310A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC32H8.09.Fd |
| Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGGATTTTACGGCTTTGTT |
| Rev primer name |
SPBC32H8.09.Rv |
| Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTAAGATGGTATGGAAATCGTT |
| Amino acid length |
483 |
| Molecular weight |
55.9 |
| Isoelectric point (calc.) |
6.7 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB?; nuclear dots;
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |