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Schizosaccharomyces pombe Postgenome Database

Gene name SPAPB1A10.11c
Gene ID 50/H06
Gene synonyms/obsolete
Gene product glutamyl-tRNA synthetase; involved in glutamyl-tRNA aminoacylation; glutamate-tRNA ligase activity
Entry clone Cloned#
ORF length (unspliced) 1581
ORF length (spliced)
Entry clone length 1581
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAPB1A10.11.Fd
Fwd primer SEQ GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGCTCTCTTATACCTCTTG
Rev primer name SPAPB1A10.11.Rv
Rev primer SEQ GGGGACCACTTTGTACAAGAAAGCTGGGTATACGATACAACTTCTTTTG
Amino acid length 526
Molecular weight 60.6
Isoelectric point (calc.) 9.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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