Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBPB8B6.03
Gene ID 50/H11
Gene synonyms/obsolete SPAP8B6.03; SPAPB8B6.03
Gene product acetamidase
Entry clone Cloned
ORF length (unspliced) 1644
ORF length (spliced)
Entry clone length 1644
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAP8B6.03.Fd
Fwd primer SEQ GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGGATACTCCGACTTGGGA
Rev primer name SPAP8B6.03.Rv
Rev primer SEQ GGGGACCACTTTGTACAAGAAAGCTGGGTAGTTGGTTAAACAATCCATT
Amino acid length 547
Molecular weight 60.5
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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