| Gene name |
SPAPB17E12.01c |
| Gene ID |
51/A06 |
| Gene synonyms/obsolete |
cox1101; cox11;
SPAC1420.04c |
| Gene product |
involved in cytochrome
c oxidase biogenesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2262 |
| ORF length (spliced) |
|
| Entry clone length |
2262 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
604T:C / 1659A:T /
1660T:A / 1661A:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAPB17E12.01.Fd |
| Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGCCCATTCTAACATGCAG |
| Rev primer name |
SPAPB17E12.01.Rv |
| Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTAGTTGAGTTTAGTTAAAAGA |
| Amino acid length |
753 |
| Molecular weight |
86.1 |
| Isoelectric point (calc.) |
9.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
2 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYSVLTDLKI |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |