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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1805.12c
Gene ID 51/A12
Gene synonyms/obsolete uep1; ubi2
Gene product ubiquitin fusion protein
Entry clone Cloned
ORF length (unspliced) 387
ORF length (spliced)
Entry clone length 387
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC1805.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAGATTTTTGTCAAGAC
Rev primer name SPAC1805.12.Rv
Rev primer SEQ AGAAAGCTGGGTACTTAAGCTTCTTCTTAGGA
Amino acid length 128
Molecular weight 14.5
Isoelectric point (calc.) 10.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear envelope; SPB; periphery; site of septum formation; nucleus>cytosol
Comments for localization observed by N-terminal tagging of GFP
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 1 images) See all images

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