Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC29A10.13
Gene ID 51/B05
Gene synonyms/obsolete atp7
Gene product F0-ATPase (subunit D); ATP synthesis coupled proton transport
Entry clone Cloned
ORF length (unspliced) 528
ORF length (spliced)
Entry clone length 528
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC29A10.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGAAAGTTGCTAGCGC
Rev primer name SPBC29A10.13.Rv
Rev primer SEQ AGAAAGCTGGGTACATAATACTCAAATCACCA
Amino acid length 175
Molecular weight 19.1
Isoelectric point (calc.) 10.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization aggregates
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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