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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC794.02
Gene ID 51/C12
Gene synonyms/obsolete wtf5; wtf4
Gene product wtf element
Entry clone Cloned
ORF length (unspliced) 1115
ORF length (spliced) 810
Entry clone length 1115
No. of intron 4
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC794.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGAATAATTACACTTC
Rev primer name SPCC794.02.Rv
Rev primer SEQ AGAAAGCTGGGTAGGCTTCACTTTCGGAGTTC
Amino acid length 269
Molecular weight 30.7
Isoelectric point (calc.) 6.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss, DeltaVision

Image information
YFP 3 images) See all images

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