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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC27D7.09c
Gene ID 51/F03
Gene synonyms/obsolete
Gene product Sp specific families; glycoprotein; possibly Sp specific; similar to Sp SPAC27D7.10c and SPAC27D7.11c and SPBC3D6.02 (paralogs); tandem duplication
Entry clone Cloned
ORF length (unspliced) 1152
ORF length (spliced)
Entry clone length 1152
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC27D7.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGCTTACTTCAATCCC
Rev primer name SPAC27D7.09.Rv
Rev primer SEQ AGAAAGCTGGGTAAACTCGTAAAGTTAATCCC
Amino acid length 383
Molecular weight 39.7
Isoelectric point (calc.) 5
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization too dark to photo
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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