Gene name |
SPAC11E3.07 |
Gene ID |
52/A03 |
Gene synonyms/obsolete |
vma4 |
Gene product |
vacuolar ATP synthase
(subunit E); V-type ATPase (E subunit); vacuolar proton pump
component; V1 sector |
Entry clone |
Cloned |
ORF length (unspliced) |
684 |
ORF length (spliced) |
|
Entry clone length |
684 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC11E3.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGTTGTCGGATGAACA |
Rev primer name |
SPAC11E3.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTATCGAACTTTCGGTTA |
Amino acid length |
227 |
Molecular weight |
25.7 |
Isoelectric point (calc.) |
5.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
vacuole membrane |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |