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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC11E3.07
Gene ID 52/A03
Gene synonyms/obsolete vma4
Gene product vacuolar ATP synthase (subunit E); V-type ATPase (E subunit); vacuolar proton pump component; V1 sector
Entry clone Cloned
ORF length (unspliced) 684
ORF length (spliced)
Entry clone length 684
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC11E3.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGTTGTCGGATGAACA
Rev primer name SPAC11E3.07.Rv
Rev primer SEQ AGAAAGCTGGGTAATTATCGAACTTTCGGTTA
Amino acid length 227
Molecular weight 25.7
Isoelectric point (calc.) 5.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) vacuole membrane
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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