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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC11E3.13c
Gene ID 52/B02
Gene synonyms/obsolete
Gene product GPI anchored protein (pers. comm. Birgit Eisenhaber); GAS family; involved in cell wall organization and biogenesis (maintenance); glycoprotein; similar to Sp SPBC342.03
Entry clone Cloned
ORF length (unspliced) 1533
ORF length (spliced)
Entry clone length 1533
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC11E3.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAACTTCCTCCATTTTTT
Rev primer name SPAC11E3.13.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCAAAAACAAGTCCGCTA
Amino acid length 510
Molecular weight 53.6
Isoelectric point (calc.) 4.1
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LHFLTTSLLL/LPYLQGLNI
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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