| Gene name |
SPMIT.05 |
| Gene ID |
52/D05 |
| Gene synonyms/obsolete |
|
| Gene product |
cob, cytochrome b,
respiratory chain complex III, similar to S. cerevisiae
cob |
| Entry clone |
Cloned in 2006
trial |
| ORF length (unspliced) |
3690 |
| ORF length (spliced) |
1164 |
| Entry clone length |
1164 |
| No. of intron |
1 |
| Sequence status |
Partially
sequenced |
| Sequence results |
100% match in both
ends |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPMIT.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAATTTTAAAATCTAA |
| Rev primer name |
SPMIT.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATCGTTTAATTGAGCTTAAA |
| Amino acid length |
|
| Molecular weight |
|
| Isoelectric point (calc.) |
|
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
|
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
|
| Localization (YFP) |
no expression
clone |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
|