 |
To download sequence files, please right-click the click button and choose "save" or "download".
All files are created using a free software "ApE".
Although you can open these files in a simple text format, we recommend to use ApE.
Attention!
When you use a series of vectors for chromosomal integration, please note that not all the transformants are proper integrants.
According to the previous paper [Genetics. 1994;136(3):849-56.], this phenomenon occurs due to gene conversion.
Although it was reported that gene conversion occurs in nearly 30% of the transformants, we estimate that this frequency is less than 10% in our pDUAL2 systems.
But we recommend to check expression of the tagged proteins before you use in your experiment.
It is enough to check two transformants in most cases.
| Gateway-incompatible vectors for chromosomal integration |
|
Plasmid name
|
Backbone
|
Promoter
|
Multicopy marker
|
Targeted locus
|
Positive selection marker
|
Sequence
|
| pLYS1U |
pUC119
|
-
|
-
|
lys1+
|
ura4+
|
|
| pLYS1U-Pnmt1 |
pUC119
|
nmt1
|
-
|
lys1+
|
ura4+
|
|
| pLYS1U-Ptub1 |
pUC119
|
tub1
|
-
|
lys1+
|
ura4+
|
|
| pLYS1K |
pUC119
|
-
|
-
|
lys1+
|
kanr
|
|
| pLYS1H |
pUC119
|
-
|
-
|
lys1+
|
hygr
|
|
| pLYS1B |
pUC119
|
-
|
-
|
lys1+
|
blar
|
|
| pHIS3U |
pUC119
|
-
|
-
|
his3+
|
ura4+
|
|
| pHIS3U-Pnmt1 |
pUC119
|
nmt1
|
-
|
his3+
|
ura4+
|
|
| pHIS3K |
pUC119
|
-
|
-
|
his3+
|
kanr
|
|
| pHIS3K-Ptub1 |
pUC119
|
tub1
|
-
|
his3+
|
kanr
|
|
| pHIS3H |
pUC119
|
-
|
-
|
his3+
|
hygr
|
|
| pHIS3B |
pUC119
|
-
|
-
|
his3+
|
blar
|
|
| pARG1U |
pUC119
|
-
|
-
|
arg1+
|
ura4+
|
|
| pARG1K |
pUC119
|
-
|
-
|
arg1+
|
kanr
|
|
| pARG1H |
pUC119
|
-
|
-
|
arg1+
|
hygr
|
|
| pARG1B |
pUC119
|
-
|
-
|
arg1+
|
blar
|
|
| pLYS1UF |
pUC119
|
-
|
ura4+
|
lys1+
|
ura4+
|
|
| pLYS1KF |
pUC119
|
-
|
ura4+
|
lys1+
|
kanr
|
|
| pLYS1HF |
pUC119
|
-
|
ura4+
|
lys1+
|
hygr
|
|
| pLYS1BF |
pUC119
|
-
|
ura4+
|
lys1+
|
blar
|
|
| pHIS3UF |
pUC119
|
-
|
ura4+
|
his3+
|
ura4+
|
|
| pHIS3KF |
pUC119
|
-
|
ura4+
|
his3+
|
kanr
|
|
| pHIS3HF |
pUC119
|
-
|
ura4+
|
his3+
|
hygr
|
|
| pHIS3BF |
pUC119
|
-
|
ura4+
|
his3+
|
blar
|
|
| pARG1UF |
pUC119
|
-
|
ura4+
|
arg1+
|
ura4+
|
|
| pARG1KF |
pUC119
|
-
|
ura4+
|
arg1+
|
kanr
|
|
| pARG1HF |
pUC119
|
-
|
ura4+
|
arg1+
|
hygr
|
|
| pARG1BF |
pUC119
|
-
|
ura4+
|
arg1+
|
blar
|
|
|
|
* Medium strength nmt1 promoter, ** Low strength nmt1 promoter
|
 |
 |
|
