Centers & Labs

RIKEN Center for Biosystems Dynamics Research

Laboratory for Translation Structural Biology

Team Leader: Takuhiro Ito (Ph.D.)
Takuhiro  Ito(Ph.D.)

We plan to conduct studies on three dimensional structures of the translation factors and/or the ribosome, mainly by X-ray crystallography and cryo-EM single particle analysis, focusing on the structural analysis of the large complexes that function in the eukaryotic translational system, in order to reveal the complicated mechanisms of eukaryotic translation.

Main Research Field

Biological Sciences

Related Research Fields

Biology

Keywords

  • X-ray crystallography
  • NMR
  • Cryo-electron microscopy
  • translation

Selected Publications

  1. Iwasaki, S., Iwasaki, W., Takahashi, M., Sakamoto, A., Watanabe, C., Shichino, Y., Floor, S.N., Fujiwara, K.,
    Mito, M., Dodo, K., Sodeoka, M., Imataka, H., Honma, T., Fukuzawa, K., Ito, T., Ingolia, N.T.:
    “The translation inhibitor Rocaglamide targets a bimolecular cavity between eIF4A and polypurine RNA”
    Molecular Cell, 73 738-748. 2018
  2. Christian, T., Sakaguchi, R., Perlinska, A.P., Lahoud, G., Ito, T., Taylor, E.A., Yokoyama, S., Sulkowska, J.I. and Hou, Y.-M.:
    "Methyl transfer by substrate signaling from a knotted protein fold"
    Nat Struct Mol Biol, 23 941-948. 2016
  3. Kashiwagi, K., Takahashi, M., Nishimoto, M., Hiyama, T.B., Higo, T., Umehara, T., Sakamoto, K., Ito, T. and Yokoyama, S.:
    "Crystal structure of eukaryotic translation initiation factor 2B"
    Nature. 531 122-125. 2016.
  4. Ito, T., Masuda, I., Yoshida, K., Goto-Ito, S., Sekine, S., Suh, S.W., Hou, Y.-M. and Yokoyama, S.:
    "Structural Basis for methyl-donor-dependent and sequence-specific binding to tRNA substrates by knotted methyltransferase TrmD"
    Proc Natl Acad Sci U S A. 112 E4197-E4205. 2015.
  5. Kuwasako, K., Takahashi, M., Unzai, S., Tsuda, K., Yoshikswa, S., He, F., Kobayashi, N., Guntert, P., Shirouzu, M., Ito, T., Tanaka, A., Yokoyama, S., Hagiwara, M., Kuroyanagi, H. and Muto, Y.:
    "RBFOX and SUP-12 sandwich a G base to cooperatively regulate tissue-specific splicing"
    Nat Struct Mol Biol. 21 778-86. 2014.
  6. Kashiwagi, K., Ito, T. and Yokoyama, S.:
    "Crystal structure of the eukaryotic translation initiation factor 2A from Schizosaccharomyces pombe"
    J Struct Funct Genomics. 15 125-130. 2014.
  7. Ito, T. and Yokoyama, S.:
    "Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions"
    Nature. 467 612-616. 2010.
  8. Ito, T., Kiyasu, N., Matsunaga, R., Takahashi, S. and Yokoyama, S.:
    "Structure of nondiscriminating glutamyl-tRNA synthetase from Thermotoga maritima."
    Acta Crystallogr D Biol Crystallogr. 66 813-820. 2010.
  9. Goto-Ito, S., Ito, T., Kuratani, M., Bessho, Y. and Yokoyama, S.:
    "Tertiary structure checkpoint at anticodon loop modification in tRNA functional maturation"
    Nat Struct Mol Biol. 16 1109-1115. 2009.
  10. Hiyama, T.B., Ito, T., Imataka, H. and Yokoyama, S.:
    "Crystal Structure of the alpha subunit of human translation initiation factor 2B"
    J Mol Biol. 392 937-951. 2009.

Lab Members

Principal Investigator

Takuhiro Ito
Team Leader

Core Members

Wakana Iwasaki
Senior Research Scientist
Kazuhiro Kashiwagi
Research Scientist
Madoka Nishimoto
Technical Scientist
Mari Takahashi
Technical Scientist
Ayako Sakamoto
Technical Scientist

Contact information

West Research Building,
1-7-22 Suehiro-cho,
Tsurumi-ku, Yokohama, Kanagawa
230-0045, Japan
Tel: +81-(0)45-503-9204
Fax: +81-(0)45-503-9460

Email: takuhiro.ito [at] riken.jp

Related links

Recent research results