Centers & Labs

RIKEN Center for Developmental Biology

Laboratory for Comparative Connectomics

Team Leader: Kazunari Miyamichi (Ph.D.)
Kazunari  Miyamichi(Ph.D.)

We use viral-genetic tools that enable mapping of input, output, and input–output relationships of specific neural types at the scale of the entire brain, and systematically map connection patterns of hypothalamic neurons underlying various social behaviors in mice. Specifically, we study sex differences in the neural circuit that regulates sexual behaviors and reproduction, and the state-dependent circuit shift for parturition and lactation in female mice during pregnancy. In addition, we extend cell-type specific viral toolbox beyond mice by using CRISPR-mediated in situ gene knock-in to study the evolution of circuit organization and function across mammalian species.

Main Research Field

Biological Sciences

Related Research Fields

Biology

Keywords

  • Neural circuit
  • Sex difference in the brain
  • Hypothalamus
  • Viral and genetic technologies

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.
  1. *Ishii, K.K.*, Osakada, T.*, Mori, H., Miyasaka, N., Yoshihara, Y., Miyamichi, K.*#, Touhara, K.#
    "A Labeled-Line Neural Circuit for Pheromone-Mediated Sexual Behaviors in Mice."
    Neuron 95, 123-137 (2017).
  2. *Beier, K.T., Steinberg, E.E., DeLoach, K.E., Xie, S., Miyamichi, K., Schwarz, L., Gao, X.J., Kremer, E.J., Malenka, R.C., Luo, L.
    "Circuit Architecture of VTA Dopamine Neurons Revealed by Systematic Input-Output Mapping."
    Cell 162, 622-34 (2015).
  3. *Schwarz, L.A.*, Miyamichi, K.*, Gao, X.J., Beier, K.T., Weissbourd, B., DeLoach, K.E., Ren, J., Ibanes, S., Malenka, R.C., Kremer, E.J., Luo, L.
    "Viral-genetic tracing of the input-output organization of a central noradrenaline circuit."
    Nature 524, 88-92 (2015).
  4. *Weissbourd, B., Ren, J., DeLoach, K.E., Guenthner, C.J., Miyamichi, K.#, Luo, L#.
    "Presynaptic partners of dorsal raphe serotonergic and GABAergic neurons."
    Neuron 83, 645-662 (2014).
  5. *Miyamichi, K.*, Shlomai-Fuchs, Y.*, Shu, M., Weissbourd, B.C., Luo, L., Mizrahi, A.
    "Dissecting local circuits: parvalbumin interneurons underlie broad feedback control of olfactory bulb output."
    Neuron 80, 1232-1245 (2013).
  6. *Guenthner, C.J., Miyamichi, K., Yang, H.H., Heller, H.C., Luo, L.
    "Permanent genetic access to transiently active neurons via TRAP: targeted recombination in active populations."
    Neuron 78, 773-784 (2013).
  7. *Miyamichi, K., Amat, F., Moussavi, F., Wang, C., Wickersham, I., Wall, N.R., Taniguchi, H., Tasic, B., Huang, Z.J., He, Z., Callaway, E.M., Horowitz, M.A., Luo, L.
    "Cortical representations of olfactory input by trans-synaptic tracing."
    Nature 472, 191-196 (2011).
  8. *Serizawa, S.*, Miyamichi, K.*, Takeuchi, H.*, Yamagishi, Y., Suzuki, M., Sakano, H.
    "A neuronal identity code for the odorant receptor-specific and activity-dependent axon sorting."
    Cell 127, 1057-1069 (2006).
  9. *Miyamichi, K.*, Serizawa, S.*, Kimura, H.M., Sakano, H.
    "Continuous and overlapping expression domains of odorant receptor genes in the olfactory epithelium determine the dorsal/ventral positioning of glomeruli in the olfactory bulb."
    J Neurosci 25, 3586-3592 (2005).
  10. *Serizawa, S.*, Miyamichi, K.*, Nakatani, H., Suzuki, M., Saito, M., Yoshihara, Y., Sakano, H.
    "Negative feedback regulation ensures the one receptor-one olfactory neuron rule in mouse."
    Science 302, 2088-94 (2003).

Contact information

5F, RIKEN CDB Bldg.C,
2-2-3 Minatojima-minamimachi, Chuo-ku
Kobe, Hyogo
650-0047 Japan

Email: Kazunari.Miyamichi [at] riken.jp

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