Centers & Labs

RIKEN Center for Developmental Biology

Laboratory for Human Organogenesis

Team Leader: Minoru Takasato (Ph.D.)
Minoru  Takasato(Ph.D.)

By precisely recapitulating the developmental processes of the human kidney in directed differentiation of human pluripotent stem cells, we are trying to achieve the ultimate goal of generating a three-dimensional kidney that is functional and can be transplanted into patients. We appreciate knowledge from basic developmental biology that is essential for such regenerative studies; therefore, we are also highly interested in studies of human embryology. Utilizing our unique technology that generates hPSC-derived kidney organoids from pluripotent stages in vitro, we are focusing particularly on uncovering the developmental mechanisms of the human mesoderm and kidney.

Main Research Field

Biology

Related Research Fields

Medicine, dentistry, and pharmacy

Keywords

  • Kidney development
  • iPS cell
  • Directed differentiation
  • Kidney organoid
  • three-dimensional organogenesis

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.
  1. *Takasato, M. et al.:
    "Generation of kidney organoids from human pluripotent stem cells."
    Nature Protocols, 11, 1681-92 (2016).
  2. *Takasato, M. & Little, M. H.:
    "A strategy for generating kidney organoids: Recapitulating the development in human pluripotent stem cells"
    Dev. Biol., 420, 210-20 (2016).
  3. *Takasato, M. et al.:
    "Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis"
    Nature 526, 564-8 (2015).
  4. *Takasato, M. & Little, M. H.:
    "The origin of the mammalian kidney: implications for recreating the kidney in vitro"
    Development 142, 1937-1947 (2015).
  5. *Takasato, M. et al.:
    "Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney"
    Nat. Cell Biol. 16, 118-26 (2014).
  6. *Hendry, C. E. et al.:
    "Direct transcriptional reprogramming of adult cells to embryonic nephron progenitors"
    J. Am. Soc. Nephrol. 24, 1424-34 (2013).
  7. *Takasato, M. et al.:
    "Trb2, a mouse homolog of tribbles, is dispensable for kidney and mouse development"
    Biochem. Biophys. Res. Commun. 373, 648-52 (2008).
  8. *Osafune, K., Takasato, M., Kispert, A., Asashima, M. & Nishinakamura, R.:
    "Identification of multipotent progenitors in the embryonic mouse kidney by a novel colony-forming assay"
    Development 133, 151-61 (2006).

Lab Members

Principal Investigator

Minoru Takasato
Team Leader

Core Members

Wei Zhao
Research Scientist
Kisa Kakiguchi
Technical Staff I
Kazuhiro Ofuji
Technical Staff I

Contact information

3F, RIKEN CDB Bldg. A,
2-2-3 Minatojima-minamimachi, Chuou-ku
Kobe, Hyogo
650-0047 Japan

Email: minoru.takasato [at] riken.jp

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