Centers & Labs

RIKEN Center for Developmental Biology

Laboratory for Organismal Patterning

Team Leader: Hiroshi Hamada (M.D., Ph.D.)
Hiroshi  Hamada(M.D., Ph.D.)

My lab studies how left-right asymmetries develop in the mouse embryo. In particular, we focus on two types of cilia that are required for left-right symmetry breaking: rotating cilia that generate leftward fluid flow, and immotile cilia that sense the fluid flow. We also study the role of maternal and paternal epigenetic regulators in embryonic and post-natal development. We address these questions by integrating live imaging, structural biology, fluid dynamics and mathematical modeling.

Main Research Field

Biological Sciences

Related Research Fields

Medicine, dentistry, and pharmacy

Keywords

  • left-right asymmetry
  • embryogenesis
  • cilia
  • Epigenetic modification of germ cell genomes
  • Aging of germ cells

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.
  1. Takaoka, K., et al.:
    "Both Nodal signaling and stochasticity select for prospective distal visceral endoderm in mouse embryos"
    Nat Commun. Accepted.
  2. Minegishi K., et al.:
    "A Wnt5 activity asymmetry and intercellular signaling polarize node cells for breaking left-right symmetry in the mouse embryo"
    Dev. Cell 40:439-452 (2017)
  3. *Shinohara K., et al.:
    "Absence of radial spokes in mouse node cilia is required for rotational movement but confers ultrastructural instability as a trade-off."
    Dev Cell 35.236-246 (2015)
  4. *Dong, F., et al.:
    "Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm."
    J. Cell. Biol. 204:203-213 (2014)
  5. *Takamatsu, A., et al.:
    "Hydrodynamic phase synchronization in mouse node cilia"
    Physical Review Letters.110:248107 (2014)
  6. *Nakamura T., et al.:
    "Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA in the mouse embryo."
    Nat Commun. 3.1322 (2012)
  7. *Yoshiba S., et al.:
    "Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2."
    Science 338.226-231 (2012)
  8. *Shinohara K., et al.:
    "Two rotating cilia in the node cavity are sufficient to break left-right symmetry in the mouse embryo."
    Nat Commun. 3.622 (2012)
  9. *Takaoka K., et al.:
    "Origin and role of distal visceral endoderm, a group of cells that determines anterior-posterior polarity of the mouse embryo."
    Nat Cell Biol 13. 743-752 (2011)
  10. *Hashimoto M., et al.:
    "Planar polarization of the node cells determines the rotation axis of the node cilia."
    Nat Cell Biol 12.170-176 (2010)

Lab Members

Principal Investigator

Hiroshi Hamada
Team Leader

Core Members

Katsutoshi Mizuno
Research Scientist
Takahiro Ide
Research Scientist
Katsura Minegishi
Research Scientist
Natsumi Shimizu
Research Scientist
Masanori Miyanishi
Research Scientist
Wang Kyaw Twan
International Program Associate
XiaoRui Cai
Technical Staff I
Eriko Kajikawa
Technical Staff I
Yayoi Ikawa
Technical Staff I
Hiromi Nishimura
Technical Staff I

Contact information

3F, RIKEN CDB Bldg.A,
2-2-3 Minatojima-minamimachi, Chuo-ku
Kobe, Hyogo
650-0047 Japan

Events

Publications