Centers & Labs

RIKEN Center for Developmental Biology

Laboratory for Vascular Morphogenesis

Team Leader: Li-Kun Phng (Ph.D.)
Li-Kun  Phng(Ph.D.)

The establishment of a blood vascular system is essential for the development of many tissues. Tissue vascularization frequently occurs through angiogenesis, which consists of many cellular processes including polarized collective cell migration, anastomosis and lumen formation.

My laboratory aims to unravel fundamental mechanisms that regulate endothelial cell dynamics and coordination during blood vessel morphogenesis. Previous studies on actin cytoskeleton revealed that specialized actin structures of different dynamics and subcellular localization drive distinct steps of vessel morphogenesis. In the future, we will 1) investigate how force regulates endothelial cell dynamics and vessel morphogenesis, and 2) identify new molecular regulators of vessel lumen formation. Our long-term goal is to understand how upstream angiogenic signals and haemodynamic forces regulate force generation in endothelial cells and their behavior.

Main Research Field

Biology

Related Research Fields

Biological Sciences

Keywords

  • Blood vessel formation
  • Endothelial cell dynamics
  • Zebrafish

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.
  1. *Gebala V, Collins R, Geudens I, Phng LK* and Gerhardt H* (*equal contribution):
    "Blood flow drives lumen formation by inverse membrane blebbing during angiogenesis in vivo"
    Nature Cell Biology 18(4), 443 - 451 (2016)
  2. *Franco CA, Jones ML, Bernabeu MO, Geudens I, Mathivet T, Rosa A, Lopes FM, Lima AP, Ragab A, Collins RT, Phng LK, Coveney PV and Gerhardt G:
    "Dynamic endothelial cell rearrangements drive developmental vessel regression"
    PLOS BIOLOGY 13(4):e1002125 (2015).
  3. *Phng LK, Gebala V, Bentley K, Philippides A, Wacker A, Mathivet T, Sauteur L, Stanchi S, HG Belting, Affolter M and Holger Gerhardt:
    "Formin-mediated actin polymerization at endothelial junctions is required for vessel lumen formation and stabilization"
    Developmental Cell 32, 123 - 132 (2015)
  4. *Phng LK*, Stanchi F and Gerhardt H* (*corresponding authors):
    "Filopodia are dispensable for endothelial tip cell guidance"
    Development 140, 4031 - 4040 (2013)
  5. *De Bock K, Georgiadou M, Schoors S, Kuchnio A, Wong BW, Cantelmo AR, Quaegebeur A, Ghesquière B, Cauwenberghs S, Eelen G, *Phng LK, Betz I, Tembuyser B, Brepoels K, Welti J, Geudens I, Segura I, Cruys B, Bifari F, Decimo I, Blanco R, Wyns S, Vangindertael J, Rocha S, Collins RT, Munck S, Daelemans D, Imamura H, Devlieger R, Rider M, Van Veldhoven PP, Schuit F, Bartrons R, Hofkens J, Fraisl P, Telang S, Deberardinis RJ, Schoonjans L, Vinckier S, Chesney J, Gerhardt H, Dewerchin M, Carmeliet P.
    "Role of PFKFB3-driven glycolysis in vessel sprouting"
    Cell 154, 651-663 (2013)
  6. *Guarani V, Deflorian G, Franco CA, Krüger M, Phng LK, Bentley K, Toussaint L, Dequiedt F, Mostoslavsky R, Schmidt MH, Zimmermann B, Brandes RP, Mione M, Westphal CH, Braun T, Zeiher AM, Gerhardt H, Dimmeler S, Potente M.
    "Acetylation-dependent regulation of endothelial Notch signalling by the SIRT1 deacetylase"
    Nature 473, 234-238 (2011)
  7. *Phng LK, Potente M, Leslie JD, Babbage J, Nyqvist D, Lobov I, Ondr JK, Rao S, Lang RA, Thurston G and Gerhardt H:
    "Nrarp coordinates endothelial Notch and Wnt signalling to control vessel density in angiogenesis"
    Developmental Cell 16, 70 - 82 (2009)
  8. *Hellström M, Phng LK, Hofmann JJ, Wallgard E, Coultas L, Lindblom P, Alva J, Nilsson AK, Karlsson L, Gaiano N, Yoon K, Rossant J, Ireula-Arispe ML, Kalén M, Gerhardt H and Betsholtz C:
    "Dll4 signalling through Notch1 regulates formation of tip cells during angiogenesis"
    Nature 445, 776 - 770 (2007)

Lab Members

Principal Investigator

Li-Kun Phng
Team Leader

Core Members

Igor Kondrychyn
Research Scientist
Akane Nomori
Technical Staff I
Wei Ting Joyce Goh
Technical Staff II
Emi Taniguchi
Assistant

Contact information

2F RIKEN CDB Building C,
2-2-3 Minatojima-minamimachi, Chuo-ku
Kobe, Hyogo
650-0047 Japan

Email: likun.phng [at] riken.jp

Related links

Events

Publications