Centers & Labs

Chief Scientist Laboratories

Chemical Genetics Laboratory

Chief Scientist: Minoru Yoshida (D.Agr.)
Minoru  Yoshida(D.Agr.)

Chemical Genetics is one of the new molecular genetics in which mutations in classical genetics are replaced by specific chemicals called bioprobes, and by which their unique cellular phenotypes are analyzed at the molecular level. This approach is providing more and more new insights into the molecular mechanisms underlying cellular response, cell cycle control and molecular recognition. In our laboratory, biological functions of a number of molecules targeted by bioprobes are studied by approaches using genomics, proteomics, and microbiology, in addition to chemical genetics. In particular, we focus on regulation of protein subcellular localization and protein post-translational modifications and their interplays such as acetylation, methylation, fatty-acylation and sumoylation. Our motto is to enjoy our research while aiming to create novel concepts in both basic and applied biologies.

Main Research Field

Biology

Related Research Fields

Chemistry

Biological Science (Molecular Biology) / Agricultural Chemistry (Applied Biochemistry) / Oncology (Tumor Biology)

Keywords

  • Chemical Biology
  • Epigenetics
  • Mode of Action
  • Transcriptional Regulation
  • Chemical Screening for Drug Discovery

Selected Publications

  1. Yoshimoto, R., Kaida, D., Furuno, M., Burroughs, A.M., Noma, S., Suzuki, H., Kawamura, Y., Hayashizaki, Y., Mayeda, A., Yoshida, M.:
    “Global analysis of pre-mRNA subcellular localization following splicing inhibition by spliceostatin A.”
    RNA 23(1), 47-57 (2017).
  2. Vo, T.V., Das, J., Meyer, M.J., Cordero, N.A., Akturk, N., Wei, X., Fair, B.J., Degatano, A.G., Fragoza, R., Liu, L.G., Matsuyama, A., Trickey, M., Horibata, S., Grimson, A., Yamano, H., Yoshida, M., Roth, F.P., Pleiss, J.A., Xia, Y., Yu, H.:
    “A Proteome-wide fission yeast interactome reveals network evolution principles from yeasts to human.”
    Cell 164, 310-323 (2016).
  3. Ito, A., Shimazu, T., Maeda, S., Shah, A.A., Tsunoda, T., Iemura, S., Natsume, T., Suzuki, T., Motohashi, H., Yamamoto, M., Yoshida, M.:
    “The subcellular localization and activity of cortactin is regulated by acetylation and interaction with Keap1.”
    Sci. Signal. 8(404), ra120 (2015).
  4. Ito, T., Umehara, T., Sasaki, K., Nakamura, Y., Nishino, N., Terada, T., Shirouzu, M., Padmanabhan, B., Yokoyama, S., Ito, A., Yoshida, M.:
    "Real-time imaging of histone H4K12-specific acetylation determines the modes of action of histone deacetylase and bromodomain inhibitors."
    Chem. Biol. 18, 495-507 (2011).
  5. Nishimura, S., Arita, Y., Honda, M., Iwamoto, K., Matsuyama, A., Shirai, A., Kawasaki, H., Kakeya, H., Kobayashi, T., Matsunaga, S., and Yoshida, M.:
    "Marine antifungal theonellamides targets 3beta-hydroxysterol to activate Rho1 signaling"
    Nature Chem. Biol. 6, 519-526 (2010).
  6. Sasaki, K., Ito, T., Nishino, N., Khochbin, S., and Yoshida, M.:
    "Real-time imaging of histone H4 hyperacetylation in living cells."
    Proc. Natl. Acad. Sci. U.S.A. 106, 16257-16262 (2009).
  7. Schneider-Poetsch, T., Usui, T., Kaida, D., and Yoshida, M.:
    "Garbled messages and corrupted translations."
    Nature Chem. Biol. 6, 189-198 (2010).
  8. Shirai, A., Matsuyama, A., Yashiroda, Y., Hashimoto, A., Kawamura, Y., Arai, R., Komatsu, Y., Horinouchi, S., and Yoshida, M.:
    "Global analysis of gel mobility of proteins and its use in target identification."
    J. Biol. Chem. 283, 10745-10752 (2008).
  9. Kaida, D., Motoyoshi, H., Tashiro, E., Nojima, T., Hagiwara, M., Ishigami, K., Watanabe, H., Kitahara, T., Yoshida, T., Nakajima, H., Tani, T., Horinouchi, S., and Yoshida, M.:
    "Spliceostatin A targets SF3b and inhibits both splicing and nuclear retention of pre-mRNA"
    Nature Chem. Biol. 3, 576-583 (2007).
  10. Matsuyama, A., Arai, R., Yashiroda, Y., Shirai, A., Kamata, A., Sekido, S., Kobayashi, Y., Hashimoto, A., Hamamoto, M., Hiraoka, Y., Horinouchi, S., and Yoshida, M.:
    "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe"
    Nature Biotechnol. 24, 841-847 (2006).

Lab Members

Principal Investigator

Minoru Yoshida
Chief Scientist

Core Members

Ryogo Hirata
Senior Research Scientist
Kenji Ogawa
Senior Research Scientist
Feng Ling
Senior Research Scientist
Ken Matsumoto
Senior Research Scientist
Akihisa Matsuyama
Senior Research Scientist
Yoko Yashiroda
Senior Research Scientist
Akihiro Ito
Senior Research Scientist
Tilman Hans Jorg Schneider-Poetsch
Research Scientist
Hiroki Kobayashi
Special Postdoctoral Researcher
Shin-ya Okamoto
Postdoctoral Researcher
Mohammad Tariq
Postdoctoral Researcher
Reina Fukushima
Junior Research Associate
Kota Noritsugu
Junior Research Associate
Asad Ali Shah
International Program Associate
Sho Yokoyama
Student Trainee
Hilbert Delos Magpantay
Student Trainee
Ayuko Noda
Student Trainee
Shiori Sugimura
Student Trainee
Kazuki Sakamoto
Student Trainee
Rumi Kurokawa
Technical Staff I
Touko Inaba
Technical Staff I
Yasue Ichikawa
Technical Staff II
Megumi Takase
Technical Staff II
Elliot Colby Bradshaw
Technical Staff II
Rei Yoshimoto
Visiting Scientist
Manabu Kawada
Visiting Scientist

Contact information

2-1 Hirosawa, Wako, Saitama 351-0198, Japan

Email: yoshidam [at] riken.jp

Related links

Annual research report