Centers & Labs

Chief Scientist Laboratories

Synthetic Cellular Chemistry Laboratory

Chief Scientist: Yukishige Ito (D.Phar.)
Yukishige  Ito(D.Phar.)

Glycoconjugates such as glycoproteins and glycolipids contain glycan chains. The latter are intimately connected with in various biological events, such as cell-cell recognition, differentiation and malignant transformation. It is becoming clear that they also play critical roles in glycoprotein activity control, stabilization, folding and degradation. Synthesis of complex and structurally diverse glycan chains is a challenging subject in synthetic organic chemistry. It is attracting a broad attention as an important area at the interface with biology. Based on these backgrounds, various attempts to synthesize glycoconjugates, particularly glycoproteins and related compounds are under current investigation, which aims at the clarification of biological roles of glycan chains. The development of new methodologies in glycan chain synthesis is another major on going subject of investigation. This program aims at establishment of fully selective and rapid assembly of glycoconjugate related glycan chains. Computational approach on glycan chains is also performed in order to clarify the conformation of biologically active oligosaccharides.

Main Research Field

Chemistry

Related Research Fields

Biology & Biochemistry

Research Subjects

  • Development of syntheic methodologies for creation of glycoconjugate-related molecules
  • Synthetic sutdies on biologically important glycoconjugates
  • Studies on intracellular functions of glycoproteins
  • Development of polysaccharide derived materials

Selected Publications

  1. Ohara, K., Takeda, Y., Daikoku, S., Hachisu, M., Seko, A., Ito, Y:
    “Profiling aglycon recognizing sites of UDP-glucose:glycoprotein glucosyltransferase by means of squarate mediated labeling”,
    Biochemistry, 54, 4909-4917 (2015)
  2. Ishiwata, A., Kaeothip, S., Takeda, Y., and Ito, Y.:
    “Synthesis of the Highly Glycosylated Hydrophilic Motif of Extensins”
    Angew. Chem. Int. Ed., 53, 9812-9816 (2014)
  3. Aikawa, J., Takeda, Y., Matsuo, I., and Ito, Y.:
    “Trimming of glucosylated N-glycans by human ER α1,2- mannosidase I”
    J. Biochem., 155, 375-384 (2014)
  4. Manabe, S., Satoh, H., Hutter, J., Lüthi, H., Laino, T., and Ito Y.:
    “Significant Substituent Effect on the Anomerization of Pyranosides: Mechanism of Anomerization and Synthesis of a 1,2-cis Glucosamine Oligomer from the 1,2-trans Anomer”
    Chem. Eur. J., 20, 124–132 (2014)
  5. Manabe, S., Yamaguchi, M., and Ito, Y.:
    “Sulfonylcarbamate as a versatile and unique hydroxy-protecting group: a protecting group stable under severe conditions and labile under mild conditions”
    Chem. Commun., 49, 8332-8334 (2013)
  6. Koizumi, A., Matsuo, I., Takatani, M., Seko, A., Hachisu, M., Takeda, Y., and Ito, Y.:
    “Top-down chemoenzymatic approach to a high-mannose-type glycan library: Synthesis of a common precursor and its enzymatic Trimming”
    Angew. Chem. Int. Ed., 52, 7426-7431 (2013)
  7. Izumi, M., Makimura, Y., Dedola, S., Seko, A., Kanamori, A., Sakono, M., Ito, Y., and Kajihara Y.:
    “Chemical synthesis of intentionally misfolded homogeneous glycoprotein: A unique approach for the study of glycoprotein quality control”
    J. Am. Chem. Soc., 134, 7238-7241 (2012)
  8. Ishiwata, A., Sakurai, A., Nishimiya, Y., Tsuda, S., and Ito, Y.:
    “Synthetic study and structural analysis of the antifreeze agent xylomannan from Upis ceramboides”
    J. Am. Chem. Soc., 133, 19524-19535 (2011)
  9. Nakagawa, Y., Doi, T., Masuda, Y., Takegoshi, K., Igarashi, Y., and Ito Y.:
    “Mapping of the primary mannose binding site of Pradimicin A”
    J. Am. Chem. Soc., 133, 17485-17493 (2011)
  10. Satoh, H., Manabe, S., Ito, Y., and Lüthi, H.:
    “Teodoro Laino, and Jürg Hutter: Endocyclic cleavage in glycosides with 2,3-trans cyclic protecting groups”
    J. Am. Chem. Soc., 133, 5610-5619 (2011)

Lab Members

Principal Investigator

Yukishige Ito
Chief Scientist

Core Members

Junichi Aikawa
Senior Research Scientist
Shino Manabe
Senior Research Scientist
Akihiro Ishiwata
Senior Research Scientist
Yasuharu Sakamoto
Senior Research Scientist
Feiqing Ding
Special Postdoctoral Researcher

Contact information

2-1 Hirosawa, Wako, Saitama 351-0198, Japan

Email: yukito [at] riken.jp

Related links

Annual research report