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RIKEN Center for Life Science Technologies

Molecular Network Control Factors Development Unit

Unit Leader: Shunsuke Tagami (Ph.D.)
Shunsuke  Tagami(Ph.D.)

This unit aims to engineer functional bio-macromolecules applicable to medical and basic biological researches, by integrating technologies in synthetic and structural biology including in vitro reconstruction of target protein complexes, in vitro evolution of protein/RNA, X-ray crystallography, and structure based protein engineering.

Research Subjects

  • We try to develop molecules that intervene in cellular pathways and thus modulate cell functions.

Selected Publications

  1. Tagami S, Attwater J, Holliger P.:
    “Simple peptides derived from the ribosomal core potentiate RNA polymerase ribozyme function”
    Nature Chemistry, 9, 325-332 (2017).
  2. Tagami S, Sekine S, Minakhin L, Esyunina D, Akasaka R, Shirouzu M, Kulbachinskiy A, Severinov K, Yokoyama S.:
    “Structural basis for promoter specificity switching of RNA polymerase by a phage factor”
    Genes Dev, 28(5), 521-531 (2014)
  3. James Attwater, Shunsuke Tagami, Michiko Kimoto, Kyle Butler, Eric T. Kool, Jesper Wengel, Piet Herdewijn, Ichiro Hirao and Philipp Holliger.:
    “Chemical fidelity of an RNA polymerase ribozyme”
    Chem Sci, 4, 2804–2814 (2013)
  4. Sekine S, Tagami S, Yokoyama S.:
    “Structural basis of transcription by bacterial and eukaryotic RNA polymerases.”
    Curr Opin Struct Biol, 22(1), 110-118 (2012)
  5. Tagami S, Sekine SI, Yokoyama S.:
    “A novel conformation of RNA polymerase sheds light on the mechanism of transcription.”
    Transcription, 2(4), 162-167 (2011)
  6. Tagami S, Sekine S, Kumarevel T, Hino N, Murayama Y, Kamegamori S, Yamamoto M, Sakamoto K, Yokoyama S.:
    “Crystal structure of bacterial RNA polymerase bound with a transcription inhibitor protein.”
    Nature, 468(7326), 978-982 (2010)
  7. Tagami S, Sekine S, Kumarevel T, Yamamoto M, Yokoyama S.:
    “Crystallization and preliminary X-ray crystallographic analysis of Thermus thermophilus transcription elongation complex bound to Gfh1.”
    Acta Crystallogr Sect F Struct Biol Cryst Commun., 66(Pt1), 63-68 (2010)

Lab Members

Principal Investigator

Shunsuke Tagami
Unit Leader

Core Members

Tomomi Sumida
Research Scientist
Kim Huynh Nhat
Postdoctoral Researcher
Misaki Moriya
Technical Staff I

Contact information

Room W223, West Building,
1-7-22 Suehiro-cho, Tsurumi-ku,
Yokohama City, Kanagawa,
230-0045, Japan
Tel: +81-(0)45-503-9459

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