Centers & Labs

RIKEN Center for Life Science Technologies

Cellular Function Conversion Technology Team

Team Leader: Harukazu Suzuki (Ph.D.)
Harukazu  Suzuki(Ph.D.)

We will develop technologies for direct cell conversion to target cell types of potential therapeutic interest, based on the integrated knowledge of the transcriptome status, the transcriptional basins, the epigenome, and the non-coding RNA, including technologies for high efficient, loci specific epigenome modification. Linked to the direct cell conversion, differentiation and safety of stem cells will be explored.

Main Research Field

Biology & Biochemistry

Related Research Fields

Molecular Biology & Genetics

Research Subjects

  • Development of cell function manipulation technologies
  • Transcription Regulation Network Analysis in Mammals
  • Promoter analysis of drug effect

Selected Publications

  1. Hon CC, Ramilowski JA, Harshbarger J, Bertin N, Rackham OJ, Gough J, Denisenko E, Schmeier S, Poulsen TM, Severin J, Lizio M, Kawaji H, Kasukawa T, Itoh M, Burroughs AM, Noma S, Djebali S, Alam T, Medvedeva YA, Testa AC, Lipovich L, Yip CW, Abugessaisa I, Mendez M, Hasegawa A, Tang D, Lassmann T, Heutink P, Babina M, Wells CA, Kojima S, Nakamura Y, Suzuki H, Daub CO, de Hoon MJ, Arner E, Hayashizaki Y, Carninci P, Forrest AR.
    "An atlas of human long non-coding RNAs with accurate 5' ends."
    Nature. 543(7644):199-204 (2017)
  2. Li JR, Suzuki T, Nishimura H, Kishima M, Maeda S, Suzuki H.
    "Asymmetric Regulation of Peripheral Genes by Two Transcriptional Regulatory Networks."
    PLoS One. 11(8):e0160459 (2016)
  3. Rackham OJ, Firas J, Fang H, Oates ME, Holmes ML, Knaupp AS; FANTOM Consortium, Suzuki H, Nefzger CM, Daub CO, Shin JW, Petretto E, Forrest AR, Hayashizaki Y, Polo JM, Gough J.
    "A predictive computational framework for direct reprogramming between human cell types."
    Nat Genet. 48(3):331-5 (2016)
  4. Murakawa Y, Yoshihara M, Kawaji H, Nishikawa M, Zayed H, Suzuki H, Fantom Consortium, Hayashizaki Y.
    "Enhanced Identification of Transcriptional Enhancers Provides Mechanistic Insights into Diseases."
    Trends Genet. 32(2):76-88 (2016)
  5. S. Roy, R. Guler, S.P. Parihar, S. Schmeier, B. Kaczkowski, H. Nishimura, J.W. Shin, Y. Negishi, M. Ozturk, R. Hurdayal, A. Kubosaki, Y. Kimura, M.J.L. de Hoon, Y. Hayashizaki, F. Brombacher, H. Suzuki.
    "Batf2/Irf1 induces inflammatory responses in classically activated macrophages, LPS and mycobacterial infection."
    J Immunol. 194(12):6035-44 (2015)
  6. Arner E, Daub CO, Vitting-Seerup K, Andersson R, Lilje B, Drablos F, Lennartsson A, Rönnerblad M, Hrydziuszko O, Vitezic M, Freeman TC, Alhendi A, Arner P, Axton R, Baillie JK, Beckhouse A, Bodega B, Briggs J, Brombacher F, Davis M, Detmar M, Ehrlund A, Endoh M, Eslami A, Fagiolini M, Fairbairn L, Faulkner GJ, Ferrai C, Fisher ME, Forrester L, Goldowitz D, Guler R, Ha T, Hara M, Herlyn M, Ikawa T, Kai C, Kawamoto H, Khachigian L, Klinken PS, Kojima S, Koseki H, Klein S, Mejhert N, Miyaguchi K, Mizuno Y, Morimoto M, Morris KJ, Mummery C, Nakachi Y, Ogishima S, Okada-Hatakeyama M, Okazaki Y, Orlando V, Ovchinnikov D, Passier R, Patrikakis M, Pombo A, Qin XY, Roy S, Sato H, Savvi S, Saxena A, Schwegmann A, Sugiyama D, Swoboda R, Tanaka H, Tomoiu A, Winteringham LN, Wolvetang E, Yanagi-Mizuochi C, Yoneda M, Zabierowski S, Zhang P, Abugessaisa I, Bertin N, Diehl AD, Fukuda S, Furuno M, Harshbarger J, Hasegawa A, Hori F, Ishikawa-Kato S, Ishizu Y, Itoh M, Kawashima T, Kojima M, Kondo N, Lizio M, Meehan TF, Mungall CJ, Murata M, Nishiyori-Sueki H, Sahin S, Sato-Nagao S, Severin J, de Hoon MJ, Kawai J, Kasukawa T, Lassmann T, Suzuki H, Kawaji H, Summers KM, Wells C; FANTOM Consortium, Hume DA, Forrest AR, Sandelin A, Carninci P, Hayashizaki Y.
    "Transcribed enhancers lead waves of coordinated transcription in transitioning mammalian cells."
    Science. 347(6225):1010-4 (2015)
  7. Tomaru Y, Hasegawa R, Suzuki T, Sato T, Kubosaki A, Suzuki M, Kawaji H, Forrest AR, Hayashizaki Y; FANTOM Consortium, Shin JW, Suzuki H.
    "A transient disruption of fibroblastic transcriptional regulatory network facilitates trans-differentiation."
    Nucleic Acids Res. 42(14):8905-13 (2014)
  8. Andersson R, Gebhard C, Miguel-Escalada I, Hoof I, Bornholdt J, Boyd M, Chen Y, Zhao X, Schmidl C, Suzuki T, Ntini E, Arner E, Valen E, Li K, Schwarzfischer L, Glatz D, Raithel J, Lilje B, Rapin N, Bagger FO, Jørgensen M, Andersen PR, Bertin N, Rackham O, Burroughs AM, Baillie JK, Ishizu Y, Shimizu Y, Furuhata E, Maeda S, Negishi Y, Mungall CJ, Meehan TF, Lassmann T, Itoh M, Kawaji H, Kondo N, Kawai J, Lennartsson A, Daub CO, Heutink P, Hume DA, Jensen TH, Suzuki H, Hayashizaki Y, Müller F; FANTOM Consortium, Forrest AR, Carninci P, Rehli M, Sandelin A.
    "An atlas of active enhancers across human cell types and tissues."
    Nature. 507(7493):455-61 (2014)
  9. The FANTOM Consortium and the RIKEN PMI and CLST (DGT)
    "A promoter-level mammalian expression atlas."
    Nature. 507(7493):462-70 (2014)
  10. Kajiyama K, Okada-Hatakeyama M, Hayashizaki H, Kawaji H, Suzuki H.
    "Capturing drug responses by quantitative promoter activity profiling."
    CPT: Pharmacometrics & Systems Pharmacology, 2:e77 (2013)

Lab Members

Principal Investigator

Harukazu Suzuki
Team Leader

Core Members

Masaaki Furuno
Senior Scientist
Jing-Ru Li
Postdoctoral Researcher
Haruka Yabukami
Technical Staff I

Contact information

1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan

Email: Harukazu [at] gsc.riken.jp

Recent research results

July 10, 2015: RIKEN RESEARCH
Uncovering the secrets of antibacterial response