Centers & Labs

RIKEN Center for Life Science Technologies

Protein Functional and Structural Biology Team

Team Leader: Mikako Shirouzu (Ph.D.)
Mikako  Shirouzu(Ph.D.)

Protein Functional and Structural Biology…We plan to develop novel methods for preparing and crystallizing important biomolecules, such as membrane proteins, and also establish the X-ray crystallography technology platform that will help to solve problems in drug development and medical treatment. By providing the three-dimensional structure information we will determine for in silico screening and by determination of the structures of target proteins complexed with candidate compounds, we will promote innovation on drug development. We also plan to determine the structures of homologous proteins, which will help the design of more specific compounds, in order to reduce side effect.

Main Research Field

Biology & Biochemistry

Related Research Fields

Molecular Biology & Genetics

Research Subjects

  • Three-dimensional structure determination for drug development
  • Development of methods for preparing difficult proteins including membrane proteins
  • Structural analysis for huge complex of biomolecules by cryo-EM

Selected Publications

  1. Jeong H, Kim JS, Song S, Shigematsu H, Yokoyama T, Hyun J, Ha NC.:
    "Pseudoatomic Structure of the Tripartite Multidrug Efflux Pump AcrAB-TolC Reveals the Intermeshing Cogwheel-like Interaction between AcrA and TolC"
    Structure, 24(2), 272-276 (2016).
  2. Furuse M, Tamogami J, Hosaka T, Kikukawa T, Shinya N, Hato M, Ohsawa N, Kim SY, Jung KH, Demura M, Miyauchi S, Kamo N, Shimono K, Kimura-Someya T, Yokoyama S, Shirouzu, M.:
    "Structural basis for the slow photocycle and late proton release in Acetabularia rhodopsin I from the marine plant Acetabularia acetabulum"
    Acta Crystallogr D Biol Crystallogr, 71(11), 2203-2216 (2015).
  3. Tokmakov AA, Kurotani A, Ikeda M, Terazawa Y, Shirouzu M, Stefanov V, Sakurai T, Yokoyama, S.:
    "Content of intrinsic disorder influences the outcome of cell-free protein synthesis"
    Sci Rep, 5:14079 (2015).
  4. Yoshida H, Park SY, Oda T, Akiyoshi T, Sato M, Shirouzu M, Tsuda K, Kuwasako K, Unzai S, Muto Y, Urano T, Obayashi E.:
    "A novel 3' splice site recognition by the two zinc fingers in the U2AF small subunit"
    Genes Dev, 29(15), 1649-1660 (2015).
  5. Tanabe H, Fujii Y, Okada-Iwabu M, Iwabu M, Nakamura Y, Hosaka T, Motoyama K, Ikeda M, Wakiyama M, Terada T, Ohsawa N, Hato M, Ogasawara S, Hino T, Murata T, Iwata S, Hirata K, Kawano Y, Yamamoto M, Kimura-Someya T, Shirouzu M, Yamauchi T, Kadowaki T, Yokoyama S.:
    "Crystal structures of the human adiponectin receptors"
    Nature, 520(7547), 312-316 (2015).
  6. Kuwasako K, Takahashi M, Unzai S, Tsuda K, Yoshikawa S, He F, Kobayashi N, Gntert P, Shirouzu M, Ito T, Tanaka A, Yokoyama S, Hagiwara M, Kuroyanagi H, Muto Y.:
    "RBFOX and SUP-12 sandwich a G base to cooperatively regulate tissue-specific splicing"
    Nat Struct Mol Biol, 21(9), 778-786 (2014).
  7. Hato M, Hosaka T, Tanabe H, Kitsuna T, Yokoyama S.:
    "A new manual dispensing system for in meso membrane protein crystallization with using a stepping motor-based dispenser"
    J Struct Funct Genomics, 15(3), 165-171 (2014).
  8. Takeo K, Tanimura S, Shinoda T, Osawa S, Zahariev IK, Takegami N, Ishizuka-Katsura Y, Shinya N, Takagi-Niidome S, Tominaga A, Ohsawa N, Kimura-Someya T, Shirouzu M, Yokoshima S, Yokoyama S, Fukuyama T, Tomita T, Iwatsubo T.:
    "Allosteric regulation of γ-secretase activity by a phenylimidazole-type γ-secretase modulator"
    Proc Natl Acad Sci U S A, 111(29), 10544-10549 (2014).
  9. Parker LJ, Taruya S, Tsuganezawa K, Ogawa N, Mikuni J, Honda K, Tomabechi Y, Handa N, Shirouzu M, Yokoyama S, Tanaka A.:
    "Kinase crystal identification and ATP-competitive inhibitor screening using the fluorescent ligand SKF86002"
    Acta Crystallogr Sect D-Biol Crystallogr, 70, 392-404 (2014).
  10. Arai S, Saijo S, Suzuki K, Mizutani K, Kakinuma Y, Ishizuka-Katsura Y, Ohsawa N, Terada T, Shirouzu M, Yokoyama S, Iwata S, Yamato I, Murata T.:
    "Rotation mechanism of Enterococcus hirae V1-ATPase based on asymmetric crystal structures"
    Nature, 493(7434), 703-707 (2013).

Lab Members

Principal Investigator

Mikako Shirouzu
Team Leader

Core Members

Chie Takemoto
Deputy Team Leader
Mutsuko Niino
Senior Scientist
Akiko Tanaka
Senior Scientist
Masakatsu Hato
Senior Scientist
Tomomi Someya
Senior Scientist
Ryo Nitta
Senior Scientist
Hideki Shigematsu
Senior Scientist
Wakana Iwasaki
Senior Research Scientist
Shisako Shoji
Research Scientist
Toshiaki Hosaka
Research Scientist
Noboru Ohsawa
Research Scientist
Takehiro Shinoda
Research Scientist
Takeshi Yokoyama
Research Scientist
Kentaro Ihara
Research Scientist
Naoki Sakai
Research Scientist
Tetsuya Hori
Research Scientist
Keisuke Ueda
Postdoctoral Researcher
Naoko Nomura
Technical Scientist
Mie Goto
Technical Scientist
Kaori Ito
Technical Scientist
Yoshiko Katsura
Technical Scientist
Miyuki Murayama
Technical Scientist
Chiemi Tsumagari
Technical Scientist
Ryogo Akasaka
Technical Scientist
Tomomi Kamo
Technical Scientist
Kazuharu Hanada
Technical Scientist
Kazushige Katsura
Technical Scientist
Mariko Ikeda
Technical Scientist
Tamami Uejima
Postdoctoral Researcher
Naomi Ohbayashi
Postdoctoral Researcher
Ayako Sakamoto
Research Associate
Kengo Tsuda
Research Associate
Masumi Mori
Technical Staff 1
Mari Aoki
Technical Staff 1
Yukako Yabuki
Technical Staff I
Yukari Nakagawa
Technical Staff I
Mio Inoue
Technical Staff 2

Contact information

1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan

Email: mikako.shirouzu [at] riken.jp

Related links

Publications