Centers & Labs

RIKEN Center for Sustainable Resource Science

Cell Factory Research Team

Team Leader: Akihiko Kondo (Ph.D.)
Akihiko  Kondo(Ph.D.)

The Cell Factory Research Team organized in fiscal year 2012 develops optimum technologies for the construction of microorganisms producing biomonomers, a material in chemical products, from plant materials. We have also reinforced our collaboration with the industrial world in order to produce those biomonomers from plant materials such as herbaceous and woody biomasses at a reasonable cost.

Main Research Field


Related Research Fields

Chemistry / Biology

Research Subjects

  • Develop a method for designing a microbial cell factory
  • Building a cell factory
  • Analysis of biomass

Selected Publications

  1. Noda S, Shirai T, Oyama S, Kondo A.:
    “Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives.”
    Metabol. Eng. in press.
  2. Osanai T, Shirai T, Iijima H, Nakaya Y, Okamoto M, Kondo A, Hirai MY.:
    “Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium.”
    Front Microbiol. 6, doi: 10.3389/fmicb.2015.01064. (2015)
  3. Iijima H, Shirai T, Okamoto M, Kondo A, Hirai MY, Osanai T.:
    “Changes in primary metabolism under light and dark conditions in response to overproduction of a response regulator RpaA in the unicellular cyanobacterium Synechocystis sp. PCC 6803.”
    Front Microbiol. 6, doi: 10.3389/fmicb.2015.00888. (2015)
  4. Sasaki K, Okamoto M, Shirai T, Tsuge Y, Teramura H, Sasaki D, Kawaguchi H, Hasunuma T, Ogino C, Matsuda F, Kikuchi J, Kondo A.:
    “Precipitate obtained following membrane separation of hydrothermally pretreated rice straw liquid revealed by 2D NMR to have high lignin content.”
    Biotechnol Biofuels. 8 doi: 10.1186/s13068-015-0273-4. (2015)
  5. Teramura H, Sasaki K, Oshima T, Aikawa S, Matsuda F, Okamoto M, Shirai T, Kawaguchi H, Ogino C, Yamasaki M, Kikuchi J, Kondo A.:
    “Changes in Lignin and Polysaccharide Components in 13 Cultivars of Rice Straw following Dilute Acid Pretreatment as Studied by Solution-State 2D 1H-13C NMR.”
    PLoS One. 10, e0128417 (2015)
  6. Noda S, Shirai T, Mochida K, Matsuda F, Oyama S, Okamoto M, Kondo A.:
    “Evaluation of Brachypodium distachyon L-Tyrosine Decarboxylase Using L-Tyrosine Over-Producing Saccharomyces cerevisiae.”
    PLoS One. 21, e0125488 (2015).
  7. Noda, S., Kawai, Y., Tanaka, T., Kondo, A.:
    “4-Vinylphenol biosynthesis from cellulose as the sole carbon source using phenolic acid decarboxylase-and tyrosine ammonia lyase-expressing Streptomyces lividans.”
    Bioresour Technol, 180, 59-65. (2015)
  8. Noda, S., Matsumoto, T., Tanaka, T., Kondo, A.:
    “Secretory production of tetrameric native full-length streptavidin with thermostability using Streptomyces lividans as a host.”
    Microb Cell Fact, 14, 5 (2015)
  9. Osanai T, Shirai T, Iijima H, Kuwahara A, Suzuki I, Kondo A, Hirai MY.:
    “Alteration of cyanobacterial sugar and amino acid metabolism by overexpression hik8, encoding a KaiC-associated histidine kinase.”
    Environ Microbiol. 17, 2430-2340. (2015)
  10. Shirai T, Matsuda F, Okamoto M, Kondo A.:
    “Evaluation of control mechanisms for Saccharomyces cerevisiae central metabolic reactions using metabolome data of eight single-gene deletion mutants.”
    Appl Microbiol Biotechnol. 97, 3569-3577. (2013)

Lab Members

Principal Investigator

Akihiko Kondo
Team Leader

Core Members

Tomokazu Shirai
Deputy Team Leader
Shuhei Noda
Special Postdoctoral Researcher
Yohei Tashiro
Special Postdoctoral Researcher
Yutaro Mori
Postdoctoral Researcher
Sachiko Ooyama
Technical Staff II
Yuka Nakaya
Technical Staff II
Ryoko Orishimo
Technical Staff II

Contact information

E804 East Research Building
1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan

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