Centers & Labs

RIKEN Center for Sustainable Resource Science

Enzyme Research Team

Team Leader: Keiji Numata (Ph.D.)
Keiji  Numata(Ph.D.)

"Enzyme Research Team" aims to create "new functional enzymes" as well as to develop "new plants and microorganisms" using organellar engineering to synthesize biopolymers such as polyamide/poly(amino acid)/peptide. One of the final goals of the laboratory is to develop the bioprocess to produce and design the biopolymers as structural materials.

Main Research Field

Engineering

Related Research Fields

Chemistry / Biological Sciences / Agricultural sciences

Keywords

  • polypeptide
  • structural protein
  • enzymatic polymerization
  • marine photosynthetic bacteria
  • Organelle engineering

Selected Publications

  1. Kosuke Tsuchiya, Keiji Numata.:
    "Chemoenzymatic synthesis of polypeptides as functional and structural materials"
    Macromolecular Bioscience, in press.
  2. Kenjiro Yazawa, Joan Gimenez-Dejoz, Hiroyasu Masunaga, Takaaki Hikima, Keiji Numata.:
    "Chemoenzymatic synthesis of a peptide containing nylon monomer units for thermally processable peptide material application"
    Polymer Chemistry, in press.
  3. Kousuke Tsuchiya, Keiji Numata.:
    "Chemoenzymatic synthesis of polypeptides containing the unnatural amino acid 2-aminoisobutyric acid"
    Chemical Communications, Volume 53, pp7318-7321, 2017.
  4. Keiji Numata, Nao Ifuku, Hiroyasu Masunaga, Takaaki Hikima, Takamasa Sakai.:
    "Silk resin with hydrated dual chemical-physical crosslinks achieves high strength and toughness"
    Biomacromolecules, Volume 6, pp 1937-1946, 2017.
  5. Ting-Yu Lin, Hiroyasu Masunaga, Ryota Sato, Ali D. Malay, Kiminori Toyooka, Takaaki Hikima, Keiji Numata.:
    "Liquid crystalline granules align in a hierarchical structure to produce spider dragline microfibrils"
    Biomacromolecules, Volume 18(4), pp1350–1355, 2017.
  6. Kousuke Tsuchiya, Hiroyasu Masunaga, Keiji Numata.:
    "Tensile reinforcement of silk films by the addition of telechelic-type polyalanine"
    Biomacromolecules, Volume 18(3), pp1002–1009, 2017.
  7. Kousuke Tsuchiya, Keiji Numata.:
    "Chemical synthesis of multiblock copolypeptides inspired by spider dragline silk proteins"
    ACS Macro Letters, Volume 6, pp103–106, 2017.
  8. Takeshi Yoshizumi, Miwa Yamada, Mieko Higuchi-Takeuchi, Ken’ichiro Matsumoto, Seiichi Taguchi, Minami Matsui, Keiji Numata.:
    "Sucrose supplementation suppressed the growth inhibition in polyhydroxyalkanoate-producing plants"
    Plant Biotechnology, Volume 34, 1 pp39-43, 2017.
  9. Chayatip Insomphun, Jo-Ann Chuah, Shingo Kobayashi, Tetsuya Fujiki, Keiji Numata.:
    "Influence of hydroxyl groups on the cell viability of polyhydroxyalkanoate (PHA) scaffolds for tissue engineering"
    ACS Biomaterials Science and Engineering, in press.
  10. Jo-Ann Chuah, Akimasa Matsugami, Fumiaki Hayashi, Keiji Numata.:
    "Self-Assembled Peptide-Based System for Mitochondrial-Targeted Gene Delivery: Functional and Structural Insights"
    Biomacromolecules, Volume 17(11), pp3547–3557, 2016.

Lab Members

Principal Investigator

Keiji Numata
Team Leader

Core Members

Takeshi Yoshizumi
Senior Research Scientist
Kosuke Tsuchiya
Senior Research Scientist
Mitsuhiro Kimura
Research Scientist
Ali Andres Defrance Malay
Research Scientist
Mieko Higuchi
Research Scientist
Neval Yilmaz
Research Scientist
Kazusato Oikawa
Research Scientist
Takaaki Miyamoto
Postdoctoral Researcher
Jo-Ann Chuah
Postdoctoral Researcher
Choon Pin Foong
Postdoctoral Researcher
Kenjiro Yazawa
Postdoctoral Researcher
Nur Alia Oktaviani
Postdoctoral Researcher
Monirul Islam Md
Postdoctoral Researcher
Hiromi Aoki
Postdoctoral Researcher
Chonprakun Thagun
Postdoctoral Researcher
Prashant Gopal Gudeangadi
Postdoctoral Researcher
Takuto Imai
Postdoctoral Researcher
Joan Gimenez Dejoz
Postdoctoral Researcher
Taku Takami
Postdoctoral Researcher
Yu Miyagi
Postdoctoral Researcher
Keiko Midorikawa
Postdoctoral Researcher
Yoko Motoda
Technical Staff I
Yoko Horii
Technical Staff I
Yuki Negishi
Technical Staff I
Ayaka Tateishi
Technical Staff I
Nao Ifuku
Technical Staff I
Ryosuke Inaba
Technical Staff I

Contact information

2-1 Hirosawa, Wako, Saitama 351-0198, Japan

Email: keiji.numata [at] riken.jp

Related links

Recent research results