Centers & Labs

RIKEN Center for Sustainable Resource Science

Chemical Biology Research Group

Group Director: Hiroyuki Osada (D.Agr.)
Hiroyuki  Osada(D.Agr.)

To promote "Chemical Biological Research" that aims to elucidate biological phenomena using chemical compounds as starting materials, chemical libraries.

Our group constructs chemical libraries through the genetic engineering of microorganisms and organic synthesis, and databases that describe the chemical and biological information of the libraries.

We explore the useful bioactive compounds in the chemical library and identify the molecular targets of bioactive compounds, and elucidate the mechanisms behind the actions of the active compounds as well.

We continue to maintain the infrastructure for advanced studies of chemical biology and sustainable resource science.

Main Research Field

Complex systems

Related Research Fields

Interdisciplinary science and engineering / Biology

Research Subjects

  • Construction of a chemical bank, “Natural Products Depository (NPDepo)”
  • Expansion of the chemical library using genetic engineering and synthetic chemistry
  • Exploration of bioactive compounds, target identification and development of the new analytical methods

Selected Publications

  1. Yun C.S., Motoyama T., and Osada H.:
    "Biosynthesis of the mycotoxin tenuazonic acid by a fungal NRPS-PKS hybrid enzyme"
    Nat. Commun. 6, 8758 (2015).
  2. Soeda Y., Yoshikawa M., Almeida O.F., Sumioka A., Maeda S., Osada H., Kondoh Y., Saito A., Miyasaka T., Kimura T., Suzuki M., Koyama H., Yoshiike Y., Sugimoto H., Ihara Y., and Takashima A.:
    "Toxic tau oligomer formation blocked by capping of cysteine residues with 1,2-dihydroxybenzene groups"
    Nat. Commun. 6, 10216 (2015).
  3. Kawamura T., Kondoh Y., Muroi M., Kawatani M.,and Osada H.:
    "A small molecule that induces reactive oxygen species via cellular glutathione depletion"
    Biochem. J. 463, 53-63 (2014).
  4. Lim C.L., Nogawa T., Uramoto M., Okano A., Hongo Y., Nakamura T., Koshino H., Takahashi S., Ibrahim D., and Osada H.:
    "RK-1355A and B, novel quinomycin derivatives isolated from a microbial metabolites fraction library based on NPPlot screening"
    J. Antibiot. 67, 323-329 (2014).
  5. Wada A., Hara S., and Osada H.:
    "Ribosome display and photo-cross-linking techniques for in vitro identification of target proteins of bioactive small molecules"
    Anal. Chem. 86, 6768-6773 (2014).
  6. Futamura Y., Kawatani M., Muroi M., Aono H., Nogawa T., and Osada H.:
    "Identification of a molecular target of a novel fungal metabolite, pyrrolizilactone, by phenotypic profiling systems"
    ChemBioChem 14, 2456-2463 (2013).
  7. Motoyama T., Hayashi T., Hirota H., Ueki M., and Osada H.:
    "Terpendole E, a kinesin Eg5 inhibitor, is a key biosynthetic intermediate of indole-diterpenes in the producing fungus Chaunopycnis alba"
    Chem. Biol., 19, 1611-1619 (2012).
  8. Miyazaki I., Simizu S., Okumura H., Takagi S., and Osada H.:
    "A small-molecule inhibitor shows that pirin regulates migration of melanoma cells"
    Nat. Chem. Biol., 6, 667-73 (2010).
  9. Muroi M., Kazami S., Noda K., Kondo H., Takayama H., Kawatani M., Usui T., and Osada H.:
    "Application of proteomic profiling based on 2D-DIGE for classification of compounds according to the mechanism of action"
    Chem. Biol., 17, 460-470 (2010).
  10. Sun Y., Hahn F., Demydchuk Y., Chettle J., Tosin M., Osada H., and Leadlay P.F.:
    "In vitro reconstruction of tetronate RK-682 biosynthesis"
    Nat. Chem. Biol,. 6, 99-101 (2010).

Lab Members

Principal Investigator

Hiroyuki Osada
Group Director

Core Members

Makoto Muroi
Senior Research Scientist
Takayuki Motoyama
Senior Research Scientist
Yasumitsu Kondoh
Senior Research Scientist
Makoto Kawatani
Senior Research Scientist
Takeshi Shimizu
Senior Research Scientist
Toshihiko Nogawa
Research Scientist
Yushi Futamura
Research Scientist
Choong Soo Yun
Research Scientist
Junnosuke Otaka
Special Postdoctoral Researcher
Kazuko Yoshida
Postdoctoral Researcher
Takeshi Kashiwa
Postdoctoral Researcher
Azhar Rasul
Visiting Researcher
Ammara Khalid
International Program Associate
Harumi Aono
Technical Staff I
Akiko Okano
Technical Staff I
Miho Tanaka
Technical Staff I
Yoko Tanaka
Technical Staff I

Contact information

Chemical Biology Building,
2-1 Hirosawa, Wako, Saitama
351-0198, Japan
Tel: +81-(0)48-467-9541
Fax: +81-(0)48-462-4669

Email: hisyo [at] riken.jp

Related links

Recent research results

October 27, 2015: Press Release
Secrets of a rice-killing fungal toxin