Centers & Labs

RIKEN Center for Sustainable Resource Science

Chemical Genomics Research Group

Group Director: Minoru Yoshida (D.Agr.)
Minoru  Yoshida(D.Agr.)

Identification of novel small molecular ligands is essential to understand diverse biological phenomena and to control the biological systems by chemical methods. This project focuses on the development of useful molecular ligands that are expected to contribute to an advance in environmental and resource sciences by employing chemical libraries that consist of microbial metabolites and/or synthetic compounds. In particular, we search into novel active compounds by constructing a variety of phenotypic screening systems using genetically modified animal, plant and yeast cells, and in vitro screening systems using various target proteins that include enzymes for metabolism and epigenetics. In addition, we construct new platforms for developing high throughput screening systems. Our goal is to identify and provide unique molecular ligands that are useful for chemical biology research aiming at exploiting new area of environmental and resource sciences.

Main Research Field

Biology

Related Research Fields

Chemistry

Keywords

  • Chemical Biology
  • Epigenetics
  • Mode of Action
  • Transcriptional Regulation
  • Chemical Screening for Drug Discovery

Selected Publications

  1. Sun, X., Hirai, G., Ueki, M., Hirota, H., Wang, Q., Hongo, Y., Nakamura, T., Hitora, Y., Takahashi, H., Sodeoka, M., Osada, H., Hamamoto, M., Yoshida, M., Yashiroda, Y.:
    "Identification of novel secreted fatty acids that regulate nitrogen catabolite repression in fission yeast."
    Sci. Rep. 6, 20856 (2016).
  2. Ito, A., Shimazu, T., Maeda, S., Shah, A.A., Tsunoda, T., Iemura, S., Natsume, T., Suzuki, T., Motohashi, H., Yamamoto, M., Yoshida, M.:
    "The subcellular localization and activity of cortactin is regulated by acetylation and interaction with Keap1."
    Sci. Signal. 8(404), ra120 (2015).
  3. Feldman, J.L., Dittenhafer-Reed, K.E., Kudo, N., Thelen, J.N., Ito, A., Yoshida, M., Denu, J.M.:
    "Kinetic and structural analysis of acyl-group selectivity provides insight into Sirtuin-catalyzed deacylation."
    Biochemistry 54(19), 3037-3050 (2015).
  4. Arita, Y., Nishimura, S., Ishitsuka, R., Kishimoto, T., Ikenouchi, J., Ishii, K., Umeda, M., Matsunaga, S., Kobayashi, T., Yoshida, M.:
    "Targeting cholesterol in a liquid-disordered environment by theonellamides modulates cell membrane order and cell shape."
    Chem. Biol. 22(5), 604-610 (2015).
  5. Nakaoka, S., Sasaki, K., Ito, A., Nakao, Y., Yoshida M.:
    "A genetically encoded FRET probe to detect intranucleosomal histone H3K9 or H3K14 acetylation using BRD4, a BET family member."
    ACS Chem Biol, DOI: 10.1021/cb501046t (2015).
  6. Nishimura, S., Tokukura, M., Ochi, J., Yoshida, M., Kakeya, H.:
    "Balance between exocytosis and endocytosis determines the efficacy of sterol-targeting antibiotics."
    Chem. Biol. 21(12), 1690-1699 (2014).
  7. Hirohama, M., Voet, A.R., Ozawa, T., Saitoh, H., Nakao, Y., Zhang, K.Y., Ito, A., Yoshida, M.:
    "Assay methods for small ubiquitin-like modifier (SUMO)-SUMO-interacting motif (SIM) interactions in vivo and in vitro using a split-luciferase complementation system."
    Anal. Biochem. 448, 92-94 (2014).
  8. Hirohama, M., Kumar, A., Fukuda, I., Matsuoka, S., Igarashi, Y., Saitoh, H., Takagi, M., Shin-Ya, K., Honda, K., Kondoh, Y., Saito, T., Nakao, Y., Osada, H., Zhang, K.Y., Yoshida, M., Ito, A.:
    "Spectomycin B1 as a novel SUMOylation inhibitor that directly binds to SUMO E2."
    ACS Chem. Biol. 8(12), 2635-42 (2013).
  9. Nishimura, S., Ishii, K., Iwamoto, K., Arita, Y., Matsunaga, S., Ohno-Iwashita, Y., Sato, S.B., Kakeya, H., Kobayashi, T., Yoshida, M.:
    "Visualization of sterol-rich membrane domains with fluorescently-labeled theonellamides."
    PLOS One 8(12), e83716 (2013).
  10. Espiritu, R.A., Matsumori, N., Murata, M., Nishimura, S., Kakeya, H., Matsunaga, S., Yoshida, M.:
    "Interaction between the marine sponge cyclic peptide theonellamide A and sterols in lipid bilayers as viewed by surface plasmon resonance and solid-state (2)H nuclear magnetic resonance."
    Biochemistry 52(14), 2410-8 (2013).

Lab Members

Principal Investigator

Minoru Yoshida
Group Director

Core Members

Ken Matsumoto
Senior Research Scientist
Akihisa Matsuyama
Senior Research Scientist
Yoko Yashiroda
Senior Research Scientist
Kazuki Sasaki
Research Scientist
Akiko Fujiwara
Special Postdoctoral Researcher
Masaki Matsuoka
Postdoctoral Researcher
Tomoshige Hiratsuka
Postdoctoral Researcher
Effendi
International Program Associate
Atsushi Hashimoto
Technical Staff II

Contact information

2-1 Hirosawa, Wako, Saitama 351-0198, Japan
Tel: +81-(0)48-467-9516
Fax: +81-(0)48-462-4676

Email: yoshidam [at] riken.jp

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