Centers & Labs

RIKEN Center for Sustainable Resource Science

Drug Discovery Seed Compounds Exploratory Unit

Unit Leader: Minoru Yoshida (D.Agr.)
Minoru  Yoshida(D.Agr.)

The mission of the Seed Compounds Exploratory Unit is to contribute to searching for active compound candidates (hit compounds) for drug targets through high throughput screening (HTS) from compound libraries and, further, to identifying hit compounds (true hits) that truly act on the targets.

Main Research Field

Chemistry

Related Research Fields

Biology

Keywords

  • Chemical Biology
  • Epigenetics
  • Mode of Action
  • Transcriptional Regulation
  • Chemical Screening for Drug Discovery

Selected Publications

  1. Matsumoto, K., Kose, S., Kuwahara, I., Yoshimura, M., Imamoto, N., Yoshida, M.:
    "Y-box protein-associated acidic protein (YBAP1/C1QBP) affects the localization and cytoplasmic functions of YB-1."
    Sci. Rep., 8, 6198 (2018)
  2. Takase, S., Kurokawa, R., Arai, D., Kanemoto Kanto, K,, Okino, T., Nakao, Y., Kushiro, T., Yoshida, M., Matsumoto, K.:
    "A quantitative shRNA screen identifies ATP1A1 as a gene that regulates cytotoxicity by aurilide B."
    Sci. Rep., 7, 2002 (2017)
  3. Piotrowski, J.S., Li, S.C., Deshpande, R., Simpkins, S.W., Nelson, J., Yashiroda, Y., Barber, J.M., Safizadeh, H., Wilson, E., Okada, H., Gebre, A.A., Kubo, K., Torres, N., Leblanc, M.B., Andrusiak, K., Okamoto, R., Yoshimura, M., van Leeuwen, J., Shirahige, K., Baryshnikova, A., Brown, G., Saito, T., Costanzo, M., Andrews, B., Ohya, Y., Osada, H., Yoshida, M., Myers, C.L., Boone, C.:
    "Functional annotation of chemical libraries across diverse biological processes."
    Nat. Chem. Biol., 13, 982-993 (2017)
  4. Nakaoka, S., Sasaki, K., Ito, A., Nakao, Y., Yoshida, M.:
    "A genetically encoded FRET probe to detect intranucleosomal histone H3K9 or H3K14 acetylation using BRD4, a BET family member."
    ACS Chem. Biol., 11, 729-33 (2016)
  5. Sun, X., Hirai, G., Ueki, M., Hirota, H., Wang, Q., Hongo, Y., Nakamura, T., Hitora, Y., Takahashi, H., Sodeoka, M., Osada, H., Hamamoto, M., Yoshida, M., Yashiroda, Y.:
    "Identification of novel secreted fatty acids that regulate nitrogen catabolite repression in fission yeast."
    Sci. Rep., 6, 20856 (2016)
  6. Ling, F., Niu, R., Hatakeyama, R., Goto, Y., Shibata, T., and Yoshida, M.:
    "ROS stimulate mitochondrial allele segregation towards homoplasmy in human cells."
    Mol. Biol. Cell, 27, 1684-93 (2016)
  7. Ito, A., Shimazu, T., Maeda, S., Shah, A.A., Tsunoda, T., Iemura, S., Natsume, T., Suzuki, T., Motohashi, H., Yamamoto, M., Yoshida, M.:
    "The subcellular localization and activity of cortactin is regulated by acetylation and interaction with Keap1."
    Sci. Signal., 8, ra120 (2015)
  8. Arita, Y., Nishimura, S., Ishitsuka, R., Kishimoto, T., Ikenouchi, J., Ishii, K., Umeda, M., Matsunaga, S., Kobayashi, T., Yoshida, M.:
    "Targeting cholesterol in a liquid-disordered environment by theonellamides modulates cell membrane order and cell shape."
    Chem. Biol., 222, 604-610 (2015)
  9. Feldman, J.L., Dittenhafer-Reed, K.E., Kudo, N., Thelen, J.N., Ito, A., Yoshida, M., Denu, J.M.:
    "Kinetic and structural basis for acyl-group selectivity and NAD(+) dependence in sirtuin-catalyzed deacylation."
    Biochemistry, 54, 3037-3050 (2015)
  10. Hirohama, M., Kumar, A., Fukuda, I., Matsuoka, S., Igarashi, Y., Saitoh, H., Takagi, M., Shin-Ya, K., Honda, K., Kondoh, Y., Saito, T., Nakao, Y., Osada, H., Zhang, K.Y., Yoshida, M., Ito, A.:
    "Spectomycin B1 as a novel SUMOylation inhibitor that directly binds to SUMO E2."
    ACS Chem. Biol., 8, 2635-2642 (2013)

Lab Members

Principal Investigator

Minoru Yoshida
Unit Leader

Core Members

Tetsuo Onuki
Deputy Unit Leader
Kenji Ogawa
Senior Visiting Scientist
Ken Matsumoto
Senior Research Scientist
Norio Kudo
Research Scientist
Kousiki Mino
Research Scientist
Hiroki Kobayashi
Research Scientist
Satoko Maeda
Technical Staff I
Iku Kuwahara
Technical Staff I
Michiru Iwashita
Technical Staff I
Haruna Nishimura
Technical Staff I
Mari Agawa
Technical Staff I
Seiji Matsuoka
Technical Staff I
Mayumi Arata
Technical Staff I
Takeshi Sonoda
Technical Staff I
Akiko Nakata
Technical Staff II
Yui Mazaki
Technical Staff II
Yasue Ichikawa
Technical Staff II
Taeko Wakana
Technical Staff II