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RIKEN Center for Sustainable Resource Science Biomolecular Characterization Unit

Unit Leader: Naoshi Dohmae (Ph.D.)

Research Summary

Naoshi  Dohmae(Ph.D.)

Our unit provides high quality structural characterization methods to the field of biological science, aiming to further understand the mechanism and action of biological molecules. We manage specialized and technical instruments including protein chemical analyses, mass spectrometry. Our challenge to research, develop and fine-tune novel characterization methods for biological molecules, is an endless yet rewarding process.

Main Research Fields

  • Biology

Related Research Fields

  • Environmental Science
  • Chemistry
  • Biological Sciences
  • Agricultural Sciences
  • Medicine, Dentistry & Pharmacy

Keywords

  • Post-translational modofocations
  • Proteomics
  • Mass spectrometry
  • Epigenomics
  • Structural characterization

Selected Publications

  • 1. Kato K, Shinoda T, Nagao R, Akimoto S, Suzuki T, Dohmae N, Chen M, Allakhverdiev SI, Shen JR, Akita F, Miyazaki N, Tomo T.:
    "Structural basis for the adaptation and function of chlorophyll f in photosystem I."
    Nat Commun. 11(1):238 (2020)
  • 2. Nakayama H, Yamauchi Y, Nobe Y, Sato K, Takahashi N, Shalev-Benami M, Isobe T, Taoka M.:
    "Method for Direct Mass-Spectrometry-Based Identification of Monomethylated RNA Nucleoside Positional Isomers and Its Application to the Analysis of Leishmania rRNA."
    Anal Chem. 91(24):15634-15643 (2019)
  • 3. Nagao R, Kato K, Suzuki T, Ifuku K, Uchiyama I, Kashino Y, Dohmae N, Akimoto S, Shen JR, Miyazaki N, Akita F.:
    "Structural basis for energy harvesting and dissipation in a diatom PSII-FCPII supercomplex."
    Nature Plants. 5:890-901 (2019)
  • 4. Nagao R, Ueno Y, Akita F, Suzuki T, Dohmae N, Akimoto S, Shen JR.:
    "Biochemical characterization of photosystem I complexes having different subunit compositions of fucoxanthin chlorophyll a/c-binding proteins in the diatom Chaetoceros gracilis."
    Photosynth Res. 140(2):141-149 (2019)
  • 5. Yoshida K, Muratani M, Araki H, Miura F, Suzuki T, Dohmae N, Katou Y, Shirahige K, Ito T, Ishii S.:
    "Mapping of histone-binding sites in histone replacement-completed spermatozoa.s."
    Nat Commun. 9(1):3885 (2018)
  • 6. Kasuya G, Nakane T, Yokoyama T, Jia Y, Inoue M, Watanabe K, Nakamura R, Nishizawa T, Kusakizako T, Tsutsumi A, Yanagisawa H, Dohmae N, Hattori M, Ichijo H, Yan Z, Kikkawa M, Shirouzu M, Ishitani R, Nureki O.:
    "Cryo-EM structures of the human volume-regulated anion channel LRRC8."
    Nat Struct Mol Biol. 25(9):797-804 (2018)
  • 7. Takahashi F, Suzuki T, Osakabe Y, Betsuyaku S, Kondo Y, Dohmae N, Fukuda H, Yamaguchi-Shinozaki K, Shinozaki K.:
    "A small peptide modulates stomatal control via abscisic acid in long-distance signalling."
    Nature. 556(7700):235-238 (2018)
  • 8. Xiao Deng, Naoshi Dohmae, Kenneth H. Nealson, Hashimoto K, Okamoto A.:
    "Multi-heme cytochromes provide a pathway for survival in energy-limited environments."
    Sci Adv. 4(2):eaao5682 (2018)
  • 9. Ferry L, Fournier A, Tsusaka T, Adelmant G, Shimazu T, Matano S, Kirsh O, Amouroux R, Dohmae N, Suzuki T, Filion GJ, Deng W, de Dieuleveult M, Fritsch L, Kudithipudi S, Jeltsch A, Leonhardt H, Hajkova P, Marto JA, Arita K, Shinkai Y, Defossez PA.:
    "Methylation of DNA Ligase 1 by G9a/GLP Recruits UHRF1 to Replicating DNA and Regulates DNA Methylation."
    Mol Cell. 67(4):550-65 (2017)
  • 10. Shihoya W, Nishizawa T, Okuta A, Tani K, Dohmae N, Fujiyoshi Y, Nureki O, Doi T.:
    "Activation mechanism of endothelin ETB receptor by endothelin-1."
    Nature. 537(7620):363-68 (2016)

Recent Research Results

Related Links

Lab Members

Principal investigator

Naoshi Dohmae
Unit Leader

Core members

Makoto Muroi
Senior Research Scientist
Hiroshi Nakayama
Senior Research Scientist
Makoto Kawatani
Senior Research Scientist
Takehiro Suzuki
Senior Technical Scientist

Contact Information

Bioscience Building,
2-1 Hirosawa,
Wako, Saitama
351-0198, Japan

Email: dohmae [at] riken.jp

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