Centers & Labs

RIKEN Quantitative Biology Center

Laboratory for Biomolecular Structure and Dynamics

Team Leader: Takanori Kigawa (Ph.D.)
Takanori  Kigawa(Ph.D.)

In actual biological cellular environment, biomolecules such as proteins are generally surrounded by high concentrations of macromolecules, so-called ‘macromolecular crowding’ and these molecules are dynamically and co-operatively working. We will elucidate cellular events at atomic resolution by analyzing structural dynamics of biomolecules in the cellular environment mainly by using NMR spectroscopy. We will also develop and improve the technologies for sample preparations, stable-isotope labeling, NMR measurements, and data analyses. In collaboration with laboratories in Computational Biology Research Core. QBiC, we will contribute to the modeling of the cell from the viewpoint of structural dynamics of biomolecules.

Main Research Field

Biology & Biochemistry

Related Research Fields

Engineering / Chemistry / Molecular Biology & Genetics / Immunology / Pharmacology & Toxicology / Plant & Animal Science / Microbiology / Computer Science

Research Subjects

  • NMR analysis of structural dynamics of biomolecules in cellular environments
  • Development of technologies for NMR measurements and data analyses
  • Development of technologies for stable-isotope labeling of proteins by using the cell-free synthesis

Selected Publications

  1. Okamura, H., Nishimura, H., Nagata, T., Kigawa, T., Watanabe, T. & Katahira, M.:
    "Accurate and molecular-size-tolerant NMR quantitation of diverse components in solution"
    Sci. Rep. 6, 21742 (2016)
  2. Kasai, T., Koshiba, S., Yokoyama, J. & Kigawa, T.:
    "Stable isotope labeling strategy based on coding theory"
    J. Biomol. NMR 63, 213-221 (2015)
  3. Numata, K., Motoda, Y., Watanabe, S., Osanai, T. & Kigawa, T.:
    "Co-expression of Two Polyhydroxyalkanoate Synthase Subunits from Synechocystis sp PCC 6803 by Cell-Free Synthesis and Their Specific Activity for Polymerization of 3-Hydroxybutyryl-Coenzyme A"
    Biochemistry 54, 1401-1407 (2015)
  4. Yamasaki, K., Kigawa, T., Seki, M., Shinozaki, K. & Yokoyama, S.:
    "DNA-binding domains of plant-specific transcription factors: structure, function, and evolution"
    Trends Plant Sci. 18, 267-276 (2013)
  5. Harada, R., Tochio, N., Kigawa, T., Sugita, Y. & Feig, M.:
    "Reduced Native State Stability in Crowded Cellular Environment Due to Protein-Protein Interactions"
    J. Am. Chem. Soc. 135, 3696-3701 (2013)
  6. Matsuda, T., Watanabe, S. & Kigawa, T.:
    "Cell-free synthesis system suitable for disulfide-containing proteins"
    Biochem. Biophys. Res. Commun. 431, 296-301 (2013)
  7. Matsuda, T., Furumoto, S., Higuchi, K., Yokoyama, J., Zhang, M., et al.:
    "Rapid biochemical synthesis of C-11-labeled single chain variable fragment antibody for immuno-PET by cell-free protein synthesis"
    Bioorganic & Medicinal Chemistry 20, 6579-6582 (2012)
  8. Akama, S., Yamamura, M. & Kigawa, T.:
    "A Multiphysics Model of In Vitro Transcription Coupling Enzymatic Reaction and Precipitation Formation"
    Biophys. J. 102, 221-230 (2012)
  9. Shin, J., Chakraborty, G., Bharatham, N., Kang, C., Tochio, N., et al.:
    "NMR Solution Structure of Human Vaccinia-related Kinase 1 (VRK1) Reveals the C-terminal Tail Essential for Its Structural Stability and Autocatalytic Activity"
    J. Biol. Chem. 286, 22131-22138 (2011)
  10. Yokoyama, J., Matsuda, T., Koshiba, S., Tochio, N. & Kigawa, T.:
    "A practical method for cell-free protein synthesis to avoid stable isotope scrambling and dilution"
    Anal. Biochem. 411, 223-229 (2011)

Lab Members

Principal Investigator

Takanori Kigawa
Team Leader

Core Members

Tsutomu Mikawa
Senior Research Scientist
Satoru Watanabe
Senior Scientist
Takuma Kasai
Research Scientist
Hideyasu Okamura
Research Scientist
Hiromasa Yagi
Research Scientist
Kohsuke Inomata
Postdoctoral Researcher
Masaomi Ikari
Research Associate
Masahiro Ito
Technical Staff I
Kae Higuchi
Research Associate
Ako Kagawa
Technical Staff I
Kazue Horio
Technical Staff I

Contact information

1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
Tel: +81-(0)45-503-9203
Fax: +81-(0)45-503-9643

Email: kigawa [at] riken.jp

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