Centers & Labs

RIKEN Innovation Center

Bovine Leukemia Virus Vaccine Laboratory

Team Leader: Katsuhiko Amimoto (Ph.D.)
Deputy Team Leader: Yoko Aida (D.V.M., Ph.D.)
Katsuhiko  Amimoto(Ph.D.) Yoko Aida

Bovine leukemia virus (BLV) infects cattle worldwide, imposing a severe economic impact on the dairy cattle industry. The purpose of this research is to develop a novel vaccine against BLV for eliminating BLV infection. This approach can be applied to other retroviruses including human immunodeficiency virus type 1 (HIV-1) and human T cell leukemia virus type I (HTLV-1) which cause acquired immunodeficiency syndrome (AIDS) and adult T-cell leukemia (ATL), respectively.
[Integrated Collaborative Research with Kyoto Biken Laboratories, Inc.]

Main Research Field

Agricultural sciences

Related Research Fields

Biological Sciences / Biology / Agricultural sciences


  • Bovine leukemia virus vaccine
  • Reverse vaccinology
  • Reduction of proviral load
  • MHC typing
  • Novel modified CD4+ T-cell epitopes

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.
  1. *Noro T., Oishi E., Kaneshige T., Yaguchi K., Amimoto K., and Shimizu M.:
    "Identification and characterization of haemagglutinin epitopes of Avibacterium paragallinarum serovar C"
    Vet Microbiol.,131, 406-413 (2008).
  2. *Ogawa Y., Oishi E., Muneta Y, Sano A., Hikono H., Shibahara T., Yagi Y., and Shimoji Y.:
    “Oral vaccination against mycoplasmal pneumonia of swine using a live Erysipelothrix rhusiopathiae vaccine strain as a vector”
    Vaccine, 27, 4543-50 (2009).
  3. Takeshima S-n., Sasaki S., Meripet P., Sugimoto Y., and Yoko Aida Y.:
    “Single nucleotide polymorphisms in the bovine MHC region of Japanese Black cattle are associated with bovine leukemia virus proviral load”
    Retrovirology.14, 24 (2017)
  4. Polat M, Moe HH., Shimogiri T, Moe KK, Takeshima S-n., and Aida Y.:
    “The first molecular epidemiological study of bovine leukemia virus infection in Myanmar cattle”
    Arch. Virol., 162, 425–437 (2017)
  5. Polat M., Takeshima S-n., Hosomichi K., Kim J., Miyasaka T., Yamada K., Arainga M., Murakami T., Matsumoto Y., Veronica de la Barra Diazd, Panei C. J., González E. T., Kanemaki M., Onuma M., Giovambattista G. and Aida Y.:
    “A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis”
    Retrovirology, 13, 4 (2016)
  6. Takeshima S-n, Sonoko Watanuki A., Ishizaki H., Matoba K., and Aida Y.:
    “Development of a direct blood-based PCR system to detect BLV provirus using CoCoMo primers”
    Arch. Virol., 161, 1539-46 (2016)
  7. Takeshima S-n., Kitamura-Muramatsu Y., Yuan Y., Saito S. and Aida Y.:
    “BLV-CoCoMo-qPCR-2: Improvements to the BLV-CoCoMo-qPCR by reducing primer degeneracy and constructing an optimal standard curve”
    Arch. Virol., 160, 1325–1332 (2015)
  8. Bai L., Otsuki H., Sato H., Kohara J., Isogai E., Takeshima S-n. and Aida Y.:
    “Identification and characterization of common B cell epitope in bovine leukemia virus via high-throughput peptide screening system in infected cattle”
    Retorovirology, 30;12(1), 106 (2015)
  9. Bai L., Takeshima S-n., Isogai E., Kohara J. and Aida Y.:
    “Novel CD8+ cytotoxic T cell epitopes in bovine leukemia virus with cattle”
    Vaccine, 33, 7194-7202 (2015)
  10. Ohno A., Takeshima S-n., Matsumoto Y. and Aida Y.:
    “Risk factors associated with increased bovine leukemia virus proviralload in infected cattle in Japan from 2012 to 2014”
    Virus Research, 210, 283-290 (2015)

Lab Members

Principal Investigator

Katsuhiko Amimoto
Team Leader

Core Members

Yoko Aida
Deputy Team Leader
Shin-nosuke Takeshima
Research Scientist
Horotaka Sato
Research Scientist
Hiroyuki Otsuki
Postdoctoral Researcher
Taichi Noro
Visiting Scientist
Atsushi Iwamoto
Visiting Scientist
Yasuko Nagai
Visiting Scientist

Contact information

Engineering Building,
2-1 Hirosawa,
Wako, Saitama
351-0198, Japan
Tel: +81-(0)48-462-4420
Fax: +81-(0)48-462-4399

Email: aida [at]

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