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Bioresource Engineering Division
Atsuo OGURA
Head of Bioresource Engineering
Atsuo OGURA (D.V.M., Ph.D.)
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Research Areas

To support the projects undertaken by the Department of Biological Systems, we develop new techniques for practical genetics, which are essential for consistent, high quality maintenance and supplies of bioresources. The techniques which are to be developed immediately include those related to nuclear transfer, establishment of stem cell lines of various origins, and cryopreservation of embryos/gametes.

Research Subject

  1. Establishment of methods for cryopreservation of embryos and germ cells
  2. Establishment of methods for microinsemination (ICSI)
  3. Establishment of methods for nuclear transfer cloning
  4. Establishment of new stem cell lines for research use

Related links

  1. RIKEN BioResource Center Website_Laboratories PageNew Window
  2. Individual Website Laboratory PageNew Window

Press release

March 31, 2009
Success in doubling of mouse growth through use of an immature embryonic cell, making possible generational change in forty four days, half of the established rate and the shortest rate among vertebrates
July 17, 2007
Gene Engineering Division of BRC, in association with Kyoto University, succeeded in the world's first separation and culturing of capsular stem cells which generate sex hormones in the mouse ovary.

RIKEN RESEARCH

November 05,2010
X marks the spot
Cloning efficiency is undermined by widespread disruption of genomic regulation resulting largely from defective expression of a single gene New Window
October 08,2010
Great leap forward
Cells sourced from adult rabbits and recast as stem cells show good potential for laboratory use New Window
June 11, 2010
Exploring technology for the preservation of genetic lines
Researchers at the RIKEN BioResource Center are storing and restoring life from preserved gene lines. New Window
May 29, 2009
Shortening the generation of generations
Immature mice father faster medical research via an assisted reproduction technique that speeds up the production of mouse models New Window
November 16, 2007
Stem cell precursors of ovarian support cells
Researchers isolate and devise a way to produce progenitors of cells essential for ovarian follicle maturation New Window
September15,2006
Reviving sperm from the dead
Research shows viable DNA can be retrieved from the sperm of frozen mice to fertilize eggs New Window

List of Selected Publications

  1. Inoue K, Kohda T, Sugimoto M, Sado T, Ogonuki N, Matoba S, Shiura H, Ikeda R, Mochida K, Fujii T, Sawai K, Otte AP, Tian XC, Yang X, Ishino F, Abe K, Ogura A:
    "Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer."
    Science 330: 496-499, 2010.
  2. Honda A, Hirose M, Hara K, Matoba S, Inoue K, Miki H, Hiura H, Kanatsu-Shinohara M, Kanai Y, Kono T, Shinohara T, Ogura A.:
    "Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cells."
    Proc Natl Acad Sci USA, 104, 12389-12394 (2007).
  3. Ogonuki, N., Mochida, K., Miki, H., Inoue, K., Fray, M., Iwaki, T., Moriwaki, K., Obata, Y., Morozumi, K., Yanagimachi, R., and Ogura A.:
    "Spermatozoa and spermatids retrieved from frozen reproductive organs or frozen whole bodies of male mice can produce normal offspring"
    Proc. Natl. Acad. Sci. USA, 103, 13098-13103 (2006).
  4. Inoue, K., Ogonuki, N., Miki, H., Hirose, M., Noda, S., Kim, J-M., Aoki, F., Miyoshi, H,, and Ogura A.:
    "Inefficient reprogramming of the hematopoietic stem cell genome following nuclear transfer"
    J. Cell Sci. 119, 1985-1991(2006).
  5. Inoue, K., Wakao, H., Ogonuki, N., Miki, H., Seino, K., Nambu-Wakao, R., Noda, S., Miyoshi, H., Koseki, H., Taniguchi, M., and Ogura, A.:
    "Generation of cloned mice by direct nuclear transfer from natural killer T cells"
    Curr. Biol. 15, 1114-1118 (2005).
  6. Ogura, A., Ogonuki, N., Miki, H., and Inoue, K.:
    "Microinsemination and nuclear transfer using male germ cells"
    Int. Rev. Cytol. 246, 189-229 (2005).
  7. Kanatsu-Shinohara, M., Inoue, K., Lee, J., Yoshimoto, M., Ogonuki, N., Miki, H., Baba, S., Kato, T., Kazuki, Y., Toyokuni, S., Oshimura, M., Heike, T., Nakahata, T., Ishino, F., Ogura, A., and Shinohara, T.:
    "Generation of pluripotent stem cells from neonatal mouse testis"
    Cell 119, 1001-1012 (2004).
  8. Fulka, J Jr., Miyashita, N., Nagai, T., and Ogura, A.:
    "Do cloned mammals skip a reprogramming step"
    Nat. Biotechnol. 22, 25-26 (2004).
  9. Ogonuki, N., Inoue, K., Yamamoto, Y., Noguchi, Y., Tanemura, K., Suzuki, O., Nakayama, H., Doi, K., Ohtomo, K., Satoh, M., Nishida, A., and Ogura, A.:
    "Early death of mice cloned from somatic cells"
    Nat. Genet. 30, 253-254 (2002).
  10. Inoue, K., Kohda, T., Lee, J., Ogonuki, N., Mochida, K., Noguchi, Y., Tanemura, K., Kaneko-Ishino, T., Ishino, F., and Ogura, A.:
    "Faithful expression of imprinted genes in cloned mice"
    Science 295, 297 (2002).

Members

Principal Investigator

Atsuo OGURA
Head

Staff Scientist

Kimiko INOUE
Senior Research Scientist
Keiji MOCHIDA
Senior Technical Scientist
Narumi OGONUKI
Senior Technical Scientist

Postdoctoral Fellow

Shogo MATOBA
Special Postdoctoral Researcher
Eiji MIZUTANI
Contract Researcher

Technical Assistant

Michiko HIROSE
Technical Staff 2
Ayumi HASEGAWA
Technical Staff 2